Developing a Transformation-Independent and Unbiased qPCR Assay to Rapidly Evaluate the Determinants of DNA Assembly Efficiency
Synthetic biology is moving in the direction of larger and more sophisticated design, which depends heavily on the efficient assembly of genetic modules. Conventional evaluation of the DNA assembly efficiency (AE) requires transformation, and the whole process requires up to 10 h and is susceptible...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-08-01
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| Series: | Engineering |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095809919307076 |