Apple phlorizin oxidation product 2 inhibits proliferation and differentiation of 3T3-L1 preadipocytes

• Main Authors: Xue Wang, Juan Wang, Lina Wei, Ching Yuan Hu, Hong Deng, Yurong Guo, Yong Hong Meng
• Format: Article
• Language: English
• Published: Elsevier 2019-11-01
• Series: Journal of Functional Foods
• Subjects: Phlorizin; Phlorizin oxidation product; Apple; 3T3-L1 preadipocytes; Differentiation
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464619304499

Phlorizin is a natural polyphenol with many bioactivities, however, its low water solubility limits its application. Phlorizin oxidation product 2 (POP2) has higher water solubility than phlorizin. However, reaction velocity and conversion rate of POP2 are low, and its bioactivity has not been elucidated. Thus, we have established a new method for preparation of POP2 with a purity of 96.8%, and the inhibitory activity of POP2 on 3T3-L1 preadipocytes was investigated. POP2 decreased adipocyte viability and inhibited the accumulation of intracellular triglycerides (TG). Furthermore, POP2 suppressed transcription of fatty acid synthase (FAS) and glucose transporter 4 (GLUT-4), led to reduce lipid accumulation. Also, POP2 down-regulated transcription and expression of PPAR γ, C/EBPα, and FAS, led to inhibition of adipocyte differentiation. An efficient method to produce POP2 was developed, and we demonstrated that POP2 reduced lipid accretion. These results are useful for potential applications of phlorizin in the food and pharmaceutical industries.