Enzymatic activity in braúna seeds subjected to thermal stress

• Main Authors: Glauciana da Mata Ataíde, Eduardo Euclydes de Lima e Borges, Andressa Vasconcelos Flores
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Maria 2016-06-01
• Series: Ciência Rural
• Subjects: α-amilase; β-amilase; glicose-6-fosfato desidrogenase; Melanoxylon braúna
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000601044&lng=en&tlng=en

ABSTRACT: To occur seed germination distinct hydrolytic enzymes work together, enabling primary root protrusion. The objective of this study was to investigate changes in the activities of α-amylase, β-amylase, and glucose-6-phosphate dehydrogenase (G6PdH) during germination of Melanoxylon brauna Schott. seeds under thermal stress. To this end, seeds of this species were germinated under constant temperature (10, 25, 30 or 40°C) and samples were collected every 24h during a 96-h period, in which the activity of the enzymes were evaluated. At 25 and 30°C, optimal temperatures for the germination of the species, the activities of α-amylase, β-amylase, and G6PdH increased with seed imbibitions. At 10 and 40°C, enzyme activities decreased, impairing the germination process.