Osteogenic effect of inflammatory macrophages loaded onto mineral bone substitute in subcutaneous implants

• Main Authors: Živković Jelena M., Najman Stevo J., Vukelić Marija Đ., Stojanović Sanja, Aleksić Milena V., Stanisavljević Milica N., Najdanović Jelena G.
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2015-01-01
• Series: Archives of Biological Sciences
• Subjects: macrophages; osteogenesis; subcutaneous implantation; mineral bone substitute; blood clot
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400020Z.pdf
• ISSN: 0354-4664; 1821-4339

We analyzed the influence of inflammatory macrophages on the osteogenic process in subcutaneous implants composed of mineral bone substitute. Thioglycollate-elicited peritoneal macrophages (TEPMs) were characterized as inflammatory. This was confirmed microscopically by the nitroblue tetrazolium (NBT) test and the production of tumor necrosis factor α (TNF-α). The implants (M-type) were made of mineral bone substitute (Bio-Oss®) mixed with TEPMs and blood clot. Implants without macrophages served as the control (C-type). Subcutaneous implantation in the interscapular area was performed on BALB/c mice. Implants were extracted after 2 and 8 weeks. In M-type implants, phagocytosis and angiogenesis were more pronounced, and osteoblast-like cells aligned onto granules of implanted material and osteoid structures can be seen. The observed higher osteocalcin and lower osteopontin immunoexpression in M-type implants when compared to the control after 8 weeks suggest a more advanced osteogenic process. Our results indicate that the presence of inflammatory macrophages in the composition of an implant may have a beneficial effect on the osteogenic process. [Projekat Ministarstva nauke Republike Srbije, br. III41017]