Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution

<p>Abstract</p> <p>Background</p> <p>The entomopathogenic anamorphic fungus <it>Beauveria bassiana </it>is currently used as a biocontrol agent (BCA) of insects. Fifty-seven <it>Beauveria bassiana </it>isolates -53 from Spain- were characterized,...

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Main Authors: Quesada-Moraga Enrique, Iturriaga Enrique A, Santiago-Álvarez Cándido, Ortiz-Urquiza Almudena, Márquez Marcela, Garrido-Jurado Inmaculada, Monte Enrique, Hermosa Rosa
Format: Article
Language:English
Published: BMC 2011-04-01
Series:BMC Microbiology
Subjects:
Online Access:http://www.biomedcentral.com/1471-2180/11/84
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spelling doaj-6822c081a52c4379983406a919fb0a602020-11-25T02:09:37ZengBMCBMC Microbiology1471-21802011-04-011118410.1186/1471-2180-11-84Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distributionQuesada-Moraga EnriqueIturriaga Enrique ASantiago-Álvarez CándidoOrtiz-Urquiza AlmudenaMárquez MarcelaGarrido-Jurado InmaculadaMonte EnriqueHermosa Rosa<p>Abstract</p> <p>Background</p> <p>The entomopathogenic anamorphic fungus <it>Beauveria bassiana </it>is currently used as a biocontrol agent (BCA) of insects. Fifty-seven <it>Beauveria bassiana </it>isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of <it>B. bassiana</it>.</p> <p>Results</p> <p>Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the <it>B. bassiana s.s</it>. (<it>sensu stricto</it>) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found.</p> <p>Conclusion</p> <p>Most Spanish <it>B. bassiana </it>isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish <it>B. bassiana </it>isolates were sampled.</p> http://www.biomedcentral.com/1471-2180/11/84Entomopathogenic fungiLSU rDNA group I intronsTranslation elongation factor geneThermal growth
collection DOAJ
language English
format Article
sources DOAJ
author Quesada-Moraga Enrique
Iturriaga Enrique A
Santiago-Álvarez Cándido
Ortiz-Urquiza Almudena
Márquez Marcela
Garrido-Jurado Inmaculada
Monte Enrique
Hermosa Rosa
spellingShingle Quesada-Moraga Enrique
Iturriaga Enrique A
Santiago-Álvarez Cándido
Ortiz-Urquiza Almudena
Márquez Marcela
Garrido-Jurado Inmaculada
Monte Enrique
Hermosa Rosa
Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution
BMC Microbiology
Entomopathogenic fungi
LSU rDNA group I introns
Translation elongation factor gene
Thermal growth
author_facet Quesada-Moraga Enrique
Iturriaga Enrique A
Santiago-Álvarez Cándido
Ortiz-Urquiza Almudena
Márquez Marcela
Garrido-Jurado Inmaculada
Monte Enrique
Hermosa Rosa
author_sort Quesada-Moraga Enrique
title Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution
title_short Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution
title_full Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution
title_fullStr Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution
title_full_unstemmed Genetic analyses place most Spanish isolates of <it>Beauveria bassiana </it>in a molecular group with word-wide distribution
title_sort genetic analyses place most spanish isolates of <it>beauveria bassiana </it>in a molecular group with word-wide distribution
publisher BMC
series BMC Microbiology
issn 1471-2180
publishDate 2011-04-01
description <p>Abstract</p> <p>Background</p> <p>The entomopathogenic anamorphic fungus <it>Beauveria bassiana </it>is currently used as a biocontrol agent (BCA) of insects. Fifty-seven <it>Beauveria bassiana </it>isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of <it>B. bassiana</it>.</p> <p>Results</p> <p>Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the <it>B. bassiana s.s</it>. (<it>sensu stricto</it>) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found.</p> <p>Conclusion</p> <p>Most Spanish <it>B. bassiana </it>isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish <it>B. bassiana </it>isolates were sampled.</p>
topic Entomopathogenic fungi
LSU rDNA group I introns
Translation elongation factor gene
Thermal growth
url http://www.biomedcentral.com/1471-2180/11/84
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