Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients

Hepatitis C virus (HCV) has been associated with high prevalence of steatosis and fibrosis. The impact of single-nucleotide polymorphisms (SNP) of patatin-like phospholipase domain-containing protein 3 (PNPLA3) on the development of steatosis and fibrosis is not clarified for Egyptian patients with...

Full description

Bibliographic Details
Main Authors: Amal M.H. Mackawy, Mohamed E.H. Badawi, Ola Megahed
Format: Article
Language:English
Published: SpringerOpen 2015-10-01
Series:Egyptian Journal of Medical Human Genetics
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1110863015000713
id doaj-681e32b8b27d4ae3b21195279f69a228
record_format Article
spelling doaj-681e32b8b27d4ae3b21195279f69a2282020-11-24T22:06:35ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302015-10-0116433334110.1016/j.ejmhg.2015.06.001Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patientsAmal M.H. Mackawy0Mohamed E.H. Badawi1Ola Megahed2Department of Medical Biochemistry, Faculty of Medicine, Zagazig University, EgyptDepartment of Internal Medicine, Faculty of Medicine, Zagazig University, EgyptDepartment of Pathology, Faculty of Medicine, Zagazig University, EgyptHepatitis C virus (HCV) has been associated with high prevalence of steatosis and fibrosis. The impact of single-nucleotide polymorphisms (SNP) of patatin-like phospholipase domain-containing protein 3 (PNPLA3) on the development of steatosis and fibrosis is not clarified for Egyptian patients with chronic hepatitis C (CHC). The aim of the study was to assess whether the PNPLA3 I148M variant predisposes to steatosis, and to progressive liver damage, as evaluated fibrosis stage in Egyptian patients with CHC. Subjects and methods: 218 CHC Egyptian patients were enrolled in the study, they were grouped by Ishak stage of fibrosis on liver biopsy into group I (fibrosis score 0 or 1; n 81), group II (fibrosis score 2 or 3; n 72) and group III (fibrosis score 4–6; n 65). DNA was amplified by polymerase chain reaction. Results: PNPLA3 genotype was not associated with metabolic parameters, including body mass index (BMI) and lipid levels, but the presence of G allele is associated with higher liver enzymes and viral loads levels. The PNPLA3 G allele was associated with the severity (stage) of fibrosis (X2 = 35.83, P = 0.000). Steatosis was detected in 40.7% of C allele carriers and 58.53% of G allele carriers. The rs738409 G allele was significantly associated with steatosis in the overall CHC patients with different fibrotic stages (X2 = 6.023, P = 0.018) odds ratio (OR) and 95% CI = 1.99 (1.146–3.47). Conclusion: Steatosis and fibrosis severity seems to be PNPLA3 I148M regulated, independently to metabolic parameters but with significant association with viral loads and liver enzymes values in Egyptian patients with CHC.http://www.sciencedirect.com/science/article/pii/S1110863015000713Chronic hepatitis C infectionSteatosisLiver fibrosisGeneticsSingle nucleotide polymorphismPatatin-like phospholipase domain-containing protein 3 (PNPLA3)
collection DOAJ
language English
format Article
sources DOAJ
author Amal M.H. Mackawy
Mohamed E.H. Badawi
Ola Megahed
spellingShingle Amal M.H. Mackawy
Mohamed E.H. Badawi
Ola Megahed
Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients
Egyptian Journal of Medical Human Genetics
Chronic hepatitis C infection
Steatosis
Liver fibrosis
Genetics
Single nucleotide polymorphism
Patatin-like phospholipase domain-containing protein 3 (PNPLA3)
author_facet Amal M.H. Mackawy
Mohamed E.H. Badawi
Ola Megahed
author_sort Amal M.H. Mackawy
title Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients
title_short Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients
title_full Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients
title_fullStr Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients
title_full_unstemmed Patatin-like phospholipase domain containing-3 gene (PNPLA3) I148M polymorphism and liver damage in chronic hepatitis C Egyptian patients
title_sort patatin-like phospholipase domain containing-3 gene (pnpla3) i148m polymorphism and liver damage in chronic hepatitis c egyptian patients
publisher SpringerOpen
series Egyptian Journal of Medical Human Genetics
issn 1110-8630
publishDate 2015-10-01
description Hepatitis C virus (HCV) has been associated with high prevalence of steatosis and fibrosis. The impact of single-nucleotide polymorphisms (SNP) of patatin-like phospholipase domain-containing protein 3 (PNPLA3) on the development of steatosis and fibrosis is not clarified for Egyptian patients with chronic hepatitis C (CHC). The aim of the study was to assess whether the PNPLA3 I148M variant predisposes to steatosis, and to progressive liver damage, as evaluated fibrosis stage in Egyptian patients with CHC. Subjects and methods: 218 CHC Egyptian patients were enrolled in the study, they were grouped by Ishak stage of fibrosis on liver biopsy into group I (fibrosis score 0 or 1; n 81), group II (fibrosis score 2 or 3; n 72) and group III (fibrosis score 4–6; n 65). DNA was amplified by polymerase chain reaction. Results: PNPLA3 genotype was not associated with metabolic parameters, including body mass index (BMI) and lipid levels, but the presence of G allele is associated with higher liver enzymes and viral loads levels. The PNPLA3 G allele was associated with the severity (stage) of fibrosis (X2 = 35.83, P = 0.000). Steatosis was detected in 40.7% of C allele carriers and 58.53% of G allele carriers. The rs738409 G allele was significantly associated with steatosis in the overall CHC patients with different fibrotic stages (X2 = 6.023, P = 0.018) odds ratio (OR) and 95% CI = 1.99 (1.146–3.47). Conclusion: Steatosis and fibrosis severity seems to be PNPLA3 I148M regulated, independently to metabolic parameters but with significant association with viral loads and liver enzymes values in Egyptian patients with CHC.
topic Chronic hepatitis C infection
Steatosis
Liver fibrosis
Genetics
Single nucleotide polymorphism
Patatin-like phospholipase domain-containing protein 3 (PNPLA3)
url http://www.sciencedirect.com/science/article/pii/S1110863015000713
work_keys_str_mv AT amalmhmackawy patatinlikephospholipasedomaincontaining3genepnpla3i148mpolymorphismandliverdamageinchronichepatitiscegyptianpatients
AT mohamedehbadawi patatinlikephospholipasedomaincontaining3genepnpla3i148mpolymorphismandliverdamageinchronichepatitiscegyptianpatients
AT olamegahed patatinlikephospholipasedomaincontaining3genepnpla3i148mpolymorphismandliverdamageinchronichepatitiscegyptianpatients
_version_ 1725823031083794432