β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo
Gouty arthritis serves as an acute reaction initiated by the deposition of monosodium urate (MSU) crystals around the joints. In this study, the anti-inflammatory effects of phytochemical β-caryophyllene on MSU crystal-induced acute gouty arthritis in vivo and in silico were explored. Through bioinf...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.651305/full |
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doaj-67f16d134cf648b89d9f10d99d4b60ca2021-04-23T08:43:25ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-04-011210.3389/fphar.2021.651305651305β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In VivoWan-Yang Li0Fan Yang1Ji-Hua Chen2Guo-Feng Ren3Xiangya School of Public Health, Central South University, Changsha, ChinaSchool of Chinese Traditional Medicine, Shandong University of Traditional Chinese Medicine, Jinan, ChinaXiangya School of Public Health, Central South University, Changsha, ChinaXiangya School of Public Health, Central South University, Changsha, ChinaGouty arthritis serves as an acute reaction initiated by the deposition of monosodium urate (MSU) crystals around the joints. In this study, the anti-inflammatory effects of phytochemical β-caryophyllene on MSU crystal-induced acute gouty arthritis in vivo and in silico were explored. Through bioinformatics methods and molecular docking, it screened the specific influence pathway of β-caryophyllene on gout. Certain methods including enzyme-linked immunosorbent assay, western blotting, and immunohistochemical staining were adopted to quantify. β-caryophyllene significantly reduced inflammation and function of ankle joints in MSU Crystals-induced gouty arthritis rats, while decreasing serum cytokine levels. Furthermore, it inhibited the expressions of NLRP3, Caspase-1, ASC, TLR4, MyD88, p65, and IL-1β in the synovial tissue so as to reduce inflammation and protect ankle joints’ function. A new research approach in which β-caryophyllene treatment to acute attacks of gout is provided through the research results.https://www.frontiersin.org/articles/10.3389/fphar.2021.651305/fullβ-caryophyllenegoutmonosodium uratenucleotide-binding oligomerization domain-like receptor protein 3nuclear factor kappa-B |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Wan-Yang Li Fan Yang Ji-Hua Chen Guo-Feng Ren |
| spellingShingle |
Wan-Yang Li Fan Yang Ji-Hua Chen Guo-Feng Ren β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo Frontiers in Pharmacology β-caryophyllene gout monosodium urate nucleotide-binding oligomerization domain-like receptor protein 3 nuclear factor kappa-B |
| author_facet |
Wan-Yang Li Fan Yang Ji-Hua Chen Guo-Feng Ren |
| author_sort |
Wan-Yang Li |
| title |
β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo |
| title_short |
β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo |
| title_full |
β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo |
| title_fullStr |
β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo |
| title_full_unstemmed |
β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo |
| title_sort |
β-caryophyllene ameliorates msu-induced gouty arthritis and inflammation through inhibiting nlrp3 and nf-κb signal pathway: in silico and in vivo |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Pharmacology |
| issn |
1663-9812 |
| publishDate |
2021-04-01 |
| description |
Gouty arthritis serves as an acute reaction initiated by the deposition of monosodium urate (MSU) crystals around the joints. In this study, the anti-inflammatory effects of phytochemical β-caryophyllene on MSU crystal-induced acute gouty arthritis in vivo and in silico were explored. Through bioinformatics methods and molecular docking, it screened the specific influence pathway of β-caryophyllene on gout. Certain methods including enzyme-linked immunosorbent assay, western blotting, and immunohistochemical staining were adopted to quantify. β-caryophyllene significantly reduced inflammation and function of ankle joints in MSU Crystals-induced gouty arthritis rats, while decreasing serum cytokine levels. Furthermore, it inhibited the expressions of NLRP3, Caspase-1, ASC, TLR4, MyD88, p65, and IL-1β in the synovial tissue so as to reduce inflammation and protect ankle joints’ function. A new research approach in which β-caryophyllene treatment to acute attacks of gout is provided through the research results. |
| topic |
β-caryophyllene gout monosodium urate nucleotide-binding oligomerization domain-like receptor protein 3 nuclear factor kappa-B |
| url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.651305/full |
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