Construction of improved tools for protein localization studies in Streptococcus pneumoniae.

Construction of improved tools for protein localization studies in Streptococcus pneumoniae.

We have constructed a set of plasmids that allow efficient expression of both N- and C-terminal fusions of proteins of interest to fluorescent proteins mCherry, Citrine, CFP and GFP in the Gram-positive pathogen Streptococcus pneumoniae. In order to improve expression of the fluorescent fusions to l...

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Bibliographic Details
Main Authors: Mafalda X Henriques, Maria João Catalão, Joana Figueiredo, João Paulo Gomes, Sergio R Filipe
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23349996/?tool=EBI

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