On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment

We propose a system that transports oocytes and measures their mechanical characteristics in an open environment using a robot integrated microfluidic chip (chip). The cells are transported through a micropillar array in the chip, and their characteristics are measured by a mechanical probe and a fo...

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Main Authors:	Kou Nakahara, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai
Format:	Article
Language:	English
Published:	MDPI AG 2015-05-01
Series:	Micromachines
Subjects:	cell mechanical characteristics cell manipulation open-chip oocyte robot
Online Access:	http://www.mdpi.com/2072-666X/6/5/648

id	doaj-67cf37605f304224b7bbf3c8a4b3ad00
record_format	Article
spelling	doaj-67cf37605f304224b7bbf3c8a4b3ad002020-11-24T21:08:47ZengMDPI AGMicromachines2072-666X2015-05-016564865910.3390/mi6050648mi6050648On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open EnvironmentKou Nakahara0Shinya Sakuma1Takeshi Hayakawa2Fumihito Arai3Department of Mechanical Science and Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8601, JapanDepartment of Mechanical Science and Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8601, JapanDepartment of Mechanical Science and Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8601, JapanDepartment of Mechanical Science and Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8601, JapanWe propose a system that transports oocytes and measures their mechanical characteristics in an open environment using a robot integrated microfluidic chip (chip). The cells are transported through a micropillar array in the chip, and their characteristics are measured by a mechanical probe and a force sensor. Because the chip has an open microchannel, important cells such as oocytes are easily introduced and collected without the risk for losing them. In addition, any bubbles trapped in the chip, which degrade the measurement precision, are easily removed. To transport the oocytes through the open microchannel, we adopt a transportation technique based on a vibration-induced flow. Under this flow, oocytes arrive at the measurement point, where their mechanical characteristics are determined. We demonstrate the introduction, transportation, measurement of mechanical characteristics, and collection of oocytes using this system.http://www.mdpi.com/2072-666X/6/5/648cell mechanical characteristicscell manipulationopen-chipoocyterobot
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kou Nakahara Shinya Sakuma Takeshi Hayakawa Fumihito Arai
spellingShingle	Kou Nakahara Shinya Sakuma Takeshi Hayakawa Fumihito Arai On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment Micromachines cell mechanical characteristics cell manipulation open-chip oocyte robot
author_facet	Kou Nakahara Shinya Sakuma Takeshi Hayakawa Fumihito Arai
author_sort	Kou Nakahara
title	On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
title_short	On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
title_full	On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
title_fullStr	On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
title_full_unstemmed	On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
title_sort	on-chip transportation and measurement of mechanical characteristics of oocytes in an open environment
publisher	MDPI AG
series	Micromachines
issn	2072-666X
publishDate	2015-05-01
description	We propose a system that transports oocytes and measures their mechanical characteristics in an open environment using a robot integrated microfluidic chip (chip). The cells are transported through a micropillar array in the chip, and their characteristics are measured by a mechanical probe and a force sensor. Because the chip has an open microchannel, important cells such as oocytes are easily introduced and collected without the risk for losing them. In addition, any bubbles trapped in the chip, which degrade the measurement precision, are easily removed. To transport the oocytes through the open microchannel, we adopt a transportation technique based on a vibration-induced flow. Under this flow, oocytes arrive at the measurement point, where their mechanical characteristics are determined. We demonstrate the introduction, transportation, measurement of mechanical characteristics, and collection of oocytes using this system.
topic	cell mechanical characteristics cell manipulation open-chip oocyte robot
url	http://www.mdpi.com/2072-666X/6/5/648
work_keys_str_mv	AT kounakahara onchiptransportationandmeasurementofmechanicalcharacteristicsofoocytesinanopenenvironment AT shinyasakuma onchiptransportationandmeasurementofmechanicalcharacteristicsofoocytesinanopenenvironment AT takeshihayakawa onchiptransportationandmeasurementofmechanicalcharacteristicsofoocytesinanopenenvironment AT fumihitoarai onchiptransportationandmeasurementofmechanicalcharacteristicsofoocytesinanopenenvironment
_version_	1716759375203270656

On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment

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