Genetic circuit characterization by inferring RNA polymerase movement and ribosome usage

Debugging a genetic circuit is frustrated by the inability to characterize parts in the context of the circuit. Here the authors use RNA-seq and ribosome profiling to take ‘snapshots’ of a large circuit in different states.

Bibliographic Details
Main Authors: Amin Espah Borujeni, Jing Zhang, Hamid Doosthosseini, Alec A. K. Nielsen, Christopher A. Voigt
Format: Article
Language:English
Published: Nature Publishing Group 2020-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-18630-2
Description
Summary:Debugging a genetic circuit is frustrated by the inability to characterize parts in the context of the circuit. Here the authors use RNA-seq and ribosome profiling to take ‘snapshots’ of a large circuit in different states.
ISSN:2041-1723