Sintesis Heptapeptida Linear (H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Oh) dengan Menggunakan Dic/Oksima sebagai Reagen Pengkopling

• Main Authors: Rani Maharani, Eka Fitri Yanti
• Format: Article
• Language: English
• Published: Faculty of Science and Technology Alauddin State Islamic University of Makassar 2016-01-01
• Series: Al-Kimia
• Subjects: TMOF; Heptapeptide; Solid-phase peptide synthesis; DIC/oxyma; Crocidolomia pavonana
• Online Access: http://journal.uin-alauddin.ac.id/index.php/al-kimia/article/view/1449

A tetrapeptide, an analog of  trypsin-modulating oostatic factor (TMOF), has been synthesised by using a method of solid-phase peptide synthesis on 2-chlorotrityl chloride resin. Fmoc strategy was applied on the synthesis. The formation of peptide bond was facilitated by DIC/oxime as coupling reagent. After all of amino acids were attached on the resin, tetrapeptidyl resin was added by a mixture of TFA:water:EDT(90:5:5) in dichloromethane to cleave the peptide.Crude peptide was purified by reverse-phase column chromatography and the purified peptide was analysed by TOF ES-MS spectroscopy.