ASSOCIATION OF POLYMORPHIC MARKERS IN GENES OF INNATE IMMUNITY IN PATIENTS WITH PERIODONTITIS AND INFLAMMATORY DISEASES OF UPPER RESPIRATORY TRACT

• Main Authors: N. G. Sarkisyan, L. V. Gankovskaya, I. A. Tuzankina, O. A. Svitich, G. I. Ron, V. N. Shershnev, O. N. Kolyadina, M. A. Dolgikh
• Format: Article
• Language: Russian
• Published: Central Research Institute for Epidemiology 2016-02-01
• Series: Журнал микробиологии, эпидемиологии и иммунобиологии
• Subjects: tlr; periodontitis; polymorphism; innate immunity; cytokines; defensins; infection
• Online Access: https://microbiol.elpub.ru/jour/article/view/13
• ISSN: 0372-9311; 2686-7613

Aim. Search of association of polymorphisms in DEFВ1, IL-10, TNF-a and TLR2 genes with development of chronic generalized periodontitis in representatives of Ural region (Caucasian race). Materials and methods. 142 patients, that were split into 3 groups, took part in the study: a group of patients with periodontitis, a group with frequent inflammatory disease of upper respiratory tract and a comparison group - healthy donors. A study of polymorphic markers was carried out: DEFB1 (-44G/C), DEFB1 (-20A/G), IL-10 (-1082 A/G), TNF-a (-308 G/A), Arg753Gln and Arg677Trp using PCR in real time mode. Results. Association of infectious pathology of upper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and Arg753Gln and Arg677Trp genes was determined. Significant differences in distribution of genotypes and alleles of genes IL-10 and TNF-a in the group of patients with periodontitis and comparison group were not detected. Conclusion. DEFB1 (-44G/C) polymorphism can be examined as a marker of periodontitis development risk.