Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis

Novel drugs for methicillin-resistant Staphylococcus aureus (MRSA) hospital- and community-acquired infections are needed because of the emergence of resistance against antibiotics. In this study, methanolic and aqueous extracts of Berberis hispanica, Crataegus oxyacantha, Cistus salviifolius, Ephed...

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Main Authors: Ikrame Zeouk, Mounyr Balouiri, Khadija Bekhti
Format: Article
Language:English
Published: Hindawi Limited 2019-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2019/1803102
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spelling doaj-670f1eba39b44d818ceac4caee2eba052021-07-02T07:33:51ZengHindawi LimitedInternational Journal of Microbiology1687-918X1687-91982019-01-01201910.1155/2019/18031021803102Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against DermatitisIkrame Zeouk0Mounyr Balouiri1Khadija Bekhti2Department of Biology, Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Laboratory of Microbial Biotechnology, B.P. 2202 Imouzzer Road, Fez, MoroccoDepartment of Biology, Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Laboratory of Microbial Biotechnology, B.P. 2202 Imouzzer Road, Fez, MoroccoDepartment of Biology, Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Laboratory of Microbial Biotechnology, B.P. 2202 Imouzzer Road, Fez, MoroccoNovel drugs for methicillin-resistant Staphylococcus aureus (MRSA) hospital- and community-acquired infections are needed because of the emergence of resistance against antibiotics. In this study, methanolic and aqueous extracts of Berberis hispanica, Crataegus oxyacantha, Cistus salviifolius, Ephedra altissima, and Lavandula dentata selected from an ethnopharmacological study to treat skin infections in Sefrou city (Center of Morocco) were tested for their antistaphylococcal activity against strains often involved in cutaneous disorders: two methicillin-resistant Staphylococcus aureus strains and one strain of Staphylococcus epidermidis using the well-diffusion assay, while the agar macrodilution method was used to determine the minimal inhibitory concentrations. The total phenolic compounds and flavonoid contents of all tested extracts were also evaluated. Three of the five methanolic extracts showed an important antibacterial activity. Berberis hispanica extract was the most active with a minimal inhibitory concentration of 04.00 mg/ml against all tested strains, followed by Cistus salviifolius and Crataegus oxyacantha extracts containing the highest amounts of total phenols (133.83 ± 9.03 and 140.67 ± 3.17 μg equivalent of gallic acid/mg of extract). However, the aqueous extracts have not shown any activity against the tested strains. The current data suggested that the most active extracts can be a good source of natural antistaphylococcal compounds and warrants further investigations to isolate bioactive molecules.http://dx.doi.org/10.1155/2019/1803102
collection DOAJ
language English
format Article
sources DOAJ
author Ikrame Zeouk
Mounyr Balouiri
Khadija Bekhti
spellingShingle Ikrame Zeouk
Mounyr Balouiri
Khadija Bekhti
Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis
International Journal of Microbiology
author_facet Ikrame Zeouk
Mounyr Balouiri
Khadija Bekhti
author_sort Ikrame Zeouk
title Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis
title_short Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis
title_full Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis
title_fullStr Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis
title_full_unstemmed Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis
title_sort antistaphylococcal activity and phytochemical analysis of crude extracts of five medicinal plants used in the center of morocco against dermatitis
publisher Hindawi Limited
series International Journal of Microbiology
issn 1687-918X
1687-9198
publishDate 2019-01-01
description Novel drugs for methicillin-resistant Staphylococcus aureus (MRSA) hospital- and community-acquired infections are needed because of the emergence of resistance against antibiotics. In this study, methanolic and aqueous extracts of Berberis hispanica, Crataegus oxyacantha, Cistus salviifolius, Ephedra altissima, and Lavandula dentata selected from an ethnopharmacological study to treat skin infections in Sefrou city (Center of Morocco) were tested for their antistaphylococcal activity against strains often involved in cutaneous disorders: two methicillin-resistant Staphylococcus aureus strains and one strain of Staphylococcus epidermidis using the well-diffusion assay, while the agar macrodilution method was used to determine the minimal inhibitory concentrations. The total phenolic compounds and flavonoid contents of all tested extracts were also evaluated. Three of the five methanolic extracts showed an important antibacterial activity. Berberis hispanica extract was the most active with a minimal inhibitory concentration of 04.00 mg/ml against all tested strains, followed by Cistus salviifolius and Crataegus oxyacantha extracts containing the highest amounts of total phenols (133.83 ± 9.03 and 140.67 ± 3.17 μg equivalent of gallic acid/mg of extract). However, the aqueous extracts have not shown any activity against the tested strains. The current data suggested that the most active extracts can be a good source of natural antistaphylococcal compounds and warrants further investigations to isolate bioactive molecules.
url http://dx.doi.org/10.1155/2019/1803102
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