ANTAGONISTIC ACTIVITY OF METABOLIС PRODUCTS OF BACTERIA LACTOBACILLUS PLANTARUM AND ENTEROCOCCUS ITALICUS WITH JOINT ACTION AGAINST PHYTOPATHOGENIC BACTERIA

• Main Authors: А. Г. Мерліч, І. Д. Жунько, Н. В. Ліманська, В. O. Іваниця
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2017-09-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: антагоністична активність; метаболіти; lactobacillus plantarum; enterococcus italicus
• Online Access: http://mbt.onu.edu.ua/article/view/110876

Aim. Detection of antagonistic activity of metabolic products of Lactobacillus plantarum and Enterococcus italicus ONU547 bacterial strains with joint action against phytopathogenic bacteria in vitro. Methods. Antagonistic activity of bacterial strains of E. italicus ONU547, L. plantarum ONU12 and L. plantarum ONU311 against phytopathogenic bacteria Rhizobium radiobacter C58, Rhizobium vitis UA6, Rhizobium rhizogenes 15834, Erwinia carotovora ZM1, Ralstonia solanacearum B-1109-УКМ, Pseudomonas syringae pv. syringae 8511 and P. syringae pv. atrofaciens D13 was studied in vitro by the agar well diffusion method. Results. The combination of metabolic products of two lactobacilli strains showed the highest inhibition level among the tested mixtures (p≤0,05) against plant pathogens Rhizobium vitis and Pseudomonas syringae pv. syringae with formation of inhibition zones with radius 7,2±0,4 and 7,8±0,4 mm. Antagonistic activity of metabolic products combinations of bacterial strains L. plantarum ОNU12 and L. plantarum ONU311 was not increased compared with the activity of separated strains in most cases. The separated strain E. italicus ONU547 did not inhibit the growth of plant pathogenic bacteria. Antagonistic activity of the studied combinations was not statistically different against Rhizobium radiobacter and Erwinia carotovora. Conclusions. All of the tested lactobacteria mixtures showed antagonistic activity with different levels against phytopathogenic bacteria used in this work because of the activity of produced organic acids.