Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods

Two-dimensional electrophoresis of plasma hemolymph proteins of several endemic Baikal amphipod species showed that, as expected, hemocyanin is the main major protein in plasma of these species. Isoforms of hemocyanins form on the gels maximum 24 separate spots with slightly different molecular weig...

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Main Authors: D.S. Bedulina, A.N. Gurkov, B.K. Baduev, E.V. Borvinskaya, M.D. Dimova, M.A. Timofeyev
Format: Article
Language:English
Published: "Vikol publishing" ST Kolesnichenko V.V. 2016-02-01
Series:Journal of Stress Physiology & Biochemistry
Subjects:
Online Access:http://www.jspb.ru/issues/2016/N1/JSPB_2016_1_74-86.pdf
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spelling doaj-66f97a3cb1624beb800fc1a28e7a2b1c2020-11-24T22:30:18Zeng"Vikol publishing" ST Kolesnichenko V.V. Journal of Stress Physiology & Biochemistry1997-08381997-08382016-02-011217486Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic AmphipodsD.S. Bedulina0A.N. Gurkov1B.K. Baduev2E.V. Borvinskaya3M.D. Dimova4M.A. Timofeyev5Institute of Biology at Irkutsk State University, 664003, Irkutsk, Karl Marx str., 1, RussiaInstitute of Biology at Irkutsk State University, 664003, Irkutsk, Karl Marx str., 1, RussiaInstitute of Biology at Irkutsk State University, 664003, Irkutsk, Karl Marx str., 1, RussiaInstitute of Biology of Karelian Research Centre, Russian Academy of Sciences, 185035, Petrozavodsk, Pushkinskaya str., RussiaInstitute of Biology at Irkutsk State University, 664003, Irkutsk, Karl Marx str., 1, RussiaInstitute of Biology at Irkutsk State University, 664003, Irkutsk, Karl Marx str., 1, RussiaTwo-dimensional electrophoresis of plasma hemolymph proteins of several endemic Baikal amphipod species showed that, as expected, hemocyanin is the main major protein in plasma of these species. Isoforms of hemocyanins form on the gels maximum 24 separate spots with slightly different molecular weight and vary by their isoelectric points. Analysis of transcript sequences of hemocyanin of Baikal amphipod Eulimnogammarus verrucosus revealed a broad heterogeneity of hemocyanin isoforms, which were divided into 2 distinct groups on their amino acid sequence and have different calculated molecular weight and isoelectric points. The β-type of hemocyanin has been described for percarid crustaceans for the first time. http://www.jspb.ru/issues/2016/N1/JSPB_2016_1_74-86.pdf2D-electriphoresisamphipodsBaikalhemocyanin
collection DOAJ
language English
format Article
sources DOAJ
author D.S. Bedulina
A.N. Gurkov
B.K. Baduev
E.V. Borvinskaya
M.D. Dimova
M.A. Timofeyev
spellingShingle D.S. Bedulina
A.N. Gurkov
B.K. Baduev
E.V. Borvinskaya
M.D. Dimova
M.A. Timofeyev
Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods
Journal of Stress Physiology & Biochemistry
2D-electriphoresis
amphipods
Baikal
hemocyanin
author_facet D.S. Bedulina
A.N. Gurkov
B.K. Baduev
E.V. Borvinskaya
M.D. Dimova
M.A. Timofeyev
author_sort D.S. Bedulina
title Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods
title_short Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods
title_full Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods
title_fullStr Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods
title_full_unstemmed Preliminary Analysis of Hemocyanins in Hemolymph Plasma of Baikal Endemic Amphipods
title_sort preliminary analysis of hemocyanins in hemolymph plasma of baikal endemic amphipods
publisher "Vikol publishing" ST Kolesnichenko V.V.
series Journal of Stress Physiology & Biochemistry
issn 1997-0838
1997-0838
publishDate 2016-02-01
description Two-dimensional electrophoresis of plasma hemolymph proteins of several endemic Baikal amphipod species showed that, as expected, hemocyanin is the main major protein in plasma of these species. Isoforms of hemocyanins form on the gels maximum 24 separate spots with slightly different molecular weight and vary by their isoelectric points. Analysis of transcript sequences of hemocyanin of Baikal amphipod Eulimnogammarus verrucosus revealed a broad heterogeneity of hemocyanin isoforms, which were divided into 2 distinct groups on their amino acid sequence and have different calculated molecular weight and isoelectric points. The β-type of hemocyanin has been described for percarid crustaceans for the first time.
topic 2D-electriphoresis
amphipods
Baikal
hemocyanin
url http://www.jspb.ru/issues/2016/N1/JSPB_2016_1_74-86.pdf
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