Antimicrobial potential of different medicinal plants against food industry pathogens

• Main Authors: Miroslava Kačániová, Jana Žiarovská, Simona Kunová, Katarí­na Rovná, Tatsiana Savitskaya, Dzmitry Hrinshpan, Veronika Valková, Lucia Galovičová, Petra Borotová, Eva Ivanišová
• Format: Article
• Language: English
• Published: HACCP Consulting 2020-07-01
• Series: Potravinarstvo
• Subjects: medicinal plants; antibacterial effect; gram-positive and gram-negative bacteria; disc diffusion method; mic
• Online Access: https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1387

Work aimed to investigate the antimicrobial activity of medicinal plants against selected species of food industry pathogens in vitro conditions. The detection of antibacterial properties was examined by the disc diffusion method and the method of the minimum inhibitory concentrations (MIC). The cultivation of microorganisms after the 24 h was performed by disc diffusion method. Petri dishes have grown at 37 °C in which the Mueller - Hinton agar and application it to the sterile paper disc impregnated with the extract.  The thickness of the resulting inhibition zone was measured with a ruler after completion of the culture. After the preparation of bacteria and extracts of certain concentrations of a subsequently added to wells microplates we use the method of the minimum inhibitory concentration (MIC) which was conducted out as the second measurement, and we took the readings absorbance spectrophotometer at 570 nm using the Glomax plate spectrophotometer. We found out, that Equisetum arvense demonstrated the largest zones of inhibition to the tested Gram-positive and Gram-negative bacteria. The greatest antimicrobial activity achieved Equisetum arvense, Urtica dioica, and Taraxacum officinale against Salmonella enterica subsp. enterica CCM 3807 and Yersinia enterocolitica CCM 5671. Equisetum arvense and Taraxacum officinale was the most effective against Escherichia coli CCM 2024 and the least effective were Tussilago farfara and Mentha piperita with using the method of minimum inhibitory concentrations.