Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification

• Main Authors: Schmieder Stefan, Weißpflog Janek, Danz Norbert, Klotzbach Udo, Sonntag Frank
• Format: Article
• Language: English
• Published: De Gruyter 2016-09-01
• Series: Current Directions in Biomedical Engineering
• Subjects: biochip; biosensor; miRNA; molecular diagnostic; SPR; surface plasmon resonance
• Online Access: http://www.degruyter.com/view/j/cdbme.2016.2.issue-1/cdbme-2016-0032/cdbme-2016-0032.xml?format=INT

MiRNAs are non-coding RNA molecules that control biological functions by reducing the translation of target proteins when binding to the mRNA. Alterations of the miRNA expression profile affect the cell metabolism, which can lead to distinctive disease patterns thus suggesting miRNA as an interesting biomarker. Here we present a SPR biosensor that utilizes disposable, injection-molded sensor chip/microfluidic hybrids combined with a lateral imaging optical system for parallel analysis of three one-dimensional spot arrays to detect miRNA-93. Using a RNA-DNA-hybrid antibody for signal enhancement we could reach a limit of detection of 10 pmol/l.