Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído Evaluation of the stability of the oxidative stress plasmatic biomarker: malondialdehyde

• Main Authors: Denise Grotto, Juliana Valentini, Silvana Boeira, Clóvis Paniz, Lucas Santa Maria, Juliana Vicentini, Angela Moro, Mariele Charão, Solange Cristina Garcia, Simone Gonçalves Cardoso
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2008-01-01
• Series: Química Nova
• Subjects: oxidative stress; malondialdehyde; stability
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000200016
• ISSN: 0100-4042; 1678-7064

<abstract language="eng">Malondialdehyde (MDA) is one of the lipid peroxidation products widely used as indicator of cellular injury. However, the short-term and the long-term stability of this biomarker remain unclear. The objective of this work was to evaluate the stability of plasmatic MDA at -20 ºC, utilizing thiobarbituric acid (TBA) as derivative in spectrophotometric and chromatographic analysis. The results showed that MDA was stable for 24 h after blood collection, was not stable when stored after alkaline hydrolysis, remained stable for 30 days after TBA derivatization and was stable for 3 days when stored after n-butanol extraction, all at -20 ºC.