The picomole determination of free and total cholesterol in cells in culture.
An enzymatic, fluorometric method for the determination of free and total cholesterol in cells in culture is presented. The method is simple, requiring one reagent that includes all of the enzymes and a second reagent that increases the pH, which enhances the fluorescence of the product. The method...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1978-05-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520407254 |