Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles
The worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a need to ev...
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MDPI AG
2019-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/9/4/584 |
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doaj-663ce6c712ce4417a7038dcf9283ebd22020-11-25T00:52:34ZengMDPI AGNanomaterials2079-49912019-04-019458410.3390/nano9040584nano9040584Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> TadpolesLauris Evariste0Laura Lagier1Patrice Gonzalez2Antoine Mottier3Florence Mouchet4Stéphanie Cadarsi5Pierre Lonchambon6Guillemine Daffe7George Chimowa8Cyril Sarrieu9Elise Ompraret10Anne-Marie Galibert11Camélia Matei Ghimbeu12Eric Pinelli13Emmanuel Flahaut14Laury Gauthier15EcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceEcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceUniv. Bordeaux, UMR EPOC CNRS 5805, Aquatic ecotoxicology team, 33120 Arcachon, FranceEcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceEcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceEcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceCIRIMAT, Université de Toulouse, CNRS, INPT, UPS, UMR CNRS-UPS-INP N°5085, Université Toulouse 3 Paul Sabatier, Bât. CIRIMAT, 118 route de Narbonne, 31062 Toulouse CEDEX 9, FranceCNRS, Universite de Bordeaux, Observatoire Aquitain des Sciences de l’Univers, UMS 2567 POREA, Allee Geoffroy Saint Hilaire, F-33615 Pessac, FranceCIRIMAT, Université de Toulouse, CNRS, INPT, UPS, UMR CNRS-UPS-INP N°5085, Université Toulouse 3 Paul Sabatier, Bât. CIRIMAT, 118 route de Narbonne, 31062 Toulouse CEDEX 9, FranceCIRIMAT, Université de Toulouse, CNRS, INPT, UPS, UMR CNRS-UPS-INP N°5085, Université Toulouse 3 Paul Sabatier, Bât. CIRIMAT, 118 route de Narbonne, 31062 Toulouse CEDEX 9, FranceCIRIMAT, Université de Toulouse, CNRS, INPT, UPS, UMR CNRS-UPS-INP N°5085, Université Toulouse 3 Paul Sabatier, Bât. CIRIMAT, 118 route de Narbonne, 31062 Toulouse CEDEX 9, FranceCIRIMAT, Université de Toulouse, CNRS, INPT, UPS, UMR CNRS-UPS-INP N°5085, Université Toulouse 3 Paul Sabatier, Bât. CIRIMAT, 118 route de Narbonne, 31062 Toulouse CEDEX 9, FranceInstitut de Science des Matériaux de Mulhouse (IS2M), UMR 7360 CNRS—UHA, 15 rue Jean Starcky, BP 2488, 68057 Mulhouse CEDEX, FranceEcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceUniv. Bordeaux, UMR EPOC CNRS 5805, Aquatic ecotoxicology team, 33120 Arcachon, FranceEcoLab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceThe worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a need to evaluate the biological and ecological risk but also to find innovative solutions leading to the production of safer materials. This work focuses on the evaluation of functional group-safety relationships regarding to graphene oxide (GO) in vivo genotoxic potential toward <i>X. laevis</i> tadpoles. For this purpose, thermal treatments in H<sub>2</sub> atmosphere were applied to produce reduced graphene oxide (rGOs) with different surface group compositions. Analysis performed indicated that GO induced disturbances in erythrocyte cell cycle leading to accumulation of cells in G0/G1 phase. Significant genotoxicity due to oxidative stress was observed in larvae exposed to low GO concentration (0.1 mg·L<sup>−1</sup>). Reduction of GO at 200 °C and 1000 °C produced a material that was no longer genotoxic at low concentrations. X-ray photoelectron spectroscopy (XPS) analysis indicated that epoxide groups may constitute a good candidate to explain the genotoxic potential of the most oxidized form of the material. Thermal reduction of GO may constitute an appropriate “safer-by-design„ strategy for the development of a safer material for environment.https://www.mdpi.com/2079-4991/9/4/584graphene oxidereduced graphene oxidemicronucleusoxidative stresssafer-by-design |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lauris Evariste Laura Lagier Patrice Gonzalez Antoine Mottier Florence Mouchet Stéphanie Cadarsi Pierre Lonchambon Guillemine Daffe George Chimowa Cyril Sarrieu Elise Ompraret Anne-Marie Galibert Camélia Matei Ghimbeu Eric Pinelli Emmanuel Flahaut Laury Gauthier |
| spellingShingle |
Lauris Evariste Laura Lagier Patrice Gonzalez Antoine Mottier Florence Mouchet Stéphanie Cadarsi Pierre Lonchambon Guillemine Daffe George Chimowa Cyril Sarrieu Elise Ompraret Anne-Marie Galibert Camélia Matei Ghimbeu Eric Pinelli Emmanuel Flahaut Laury Gauthier Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles Nanomaterials graphene oxide reduced graphene oxide micronucleus oxidative stress safer-by-design |
| author_facet |
Lauris Evariste Laura Lagier Patrice Gonzalez Antoine Mottier Florence Mouchet Stéphanie Cadarsi Pierre Lonchambon Guillemine Daffe George Chimowa Cyril Sarrieu Elise Ompraret Anne-Marie Galibert Camélia Matei Ghimbeu Eric Pinelli Emmanuel Flahaut Laury Gauthier |
| author_sort |
Lauris Evariste |
| title |
Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles |
| title_short |
Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles |
| title_full |
Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles |
| title_fullStr |
Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles |
| title_full_unstemmed |
Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward <i>Xenopus laevis</i> Tadpoles |
| title_sort |
thermal reduction of graphene oxide mitigates its in vivo genotoxicity toward <i>xenopus laevis</i> tadpoles |
| publisher |
MDPI AG |
| series |
Nanomaterials |
| issn |
2079-4991 |
| publishDate |
2019-04-01 |
| description |
The worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a need to evaluate the biological and ecological risk but also to find innovative solutions leading to the production of safer materials. This work focuses on the evaluation of functional group-safety relationships regarding to graphene oxide (GO) in vivo genotoxic potential toward <i>X. laevis</i> tadpoles. For this purpose, thermal treatments in H<sub>2</sub> atmosphere were applied to produce reduced graphene oxide (rGOs) with different surface group compositions. Analysis performed indicated that GO induced disturbances in erythrocyte cell cycle leading to accumulation of cells in G0/G1 phase. Significant genotoxicity due to oxidative stress was observed in larvae exposed to low GO concentration (0.1 mg·L<sup>−1</sup>). Reduction of GO at 200 °C and 1000 °C produced a material that was no longer genotoxic at low concentrations. X-ray photoelectron spectroscopy (XPS) analysis indicated that epoxide groups may constitute a good candidate to explain the genotoxic potential of the most oxidized form of the material. Thermal reduction of GO may constitute an appropriate “safer-by-design„ strategy for the development of a safer material for environment. |
| topic |
graphene oxide reduced graphene oxide micronucleus oxidative stress safer-by-design |
| url |
https://www.mdpi.com/2079-4991/9/4/584 |
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