Identification and characterization of genetic variation in the promoter region of bovine growth hormone receptor (GHR) gene in the local cattle of Kohkiloye Va Boyer Ahmad province

• Main Authors: Mostafa Mohaqqeq Dolat Abadi, Javad Habibi Zad, Farahnaz Imani Khah
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2014-05-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: ghr- promoter region- microsatellite- transcription factors- local cattle
• Online Access: https://jab.uk.ac.ir/article_1298_ca3f753018d11d5e25900b89d8e87fc4.pdf

The growth hormone receptor (<em>GHR</em>) is a cell surface receptor for growth hormone (GH) that is required for GH to carry out its effects on target tissues. Therefore, the aim of this study was to identify and analysis of SNPs in the promoter region of GHR gene in Iranian local cattle breed (Bos taurus). The part of promoter region of GHR gene was screened by single strand conformation polymorphism (SSCP) method and DNA sequencing. A total of 3 distinct SSCP patterns were observed which further revealed an A/G transition at position -154 and two length of TG microsatellite (11 and 17 TG repeats)  upon sequence analysis in amplified fragment. The genotype frequencies of AA (TG_17/17), G (TG_11/11) and GA (TG_17/11) were 0.34, 0.24 and 0.52 respectively. In silico analysis has been shown that the single nucleotide polymorphism (SNP) at position -154 is very close to the putative binding site for C/EBP transcription factor. The possible functional activity of identified genetic variation should be approved using gene expression analysis.