In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device

The feasibility of in vivo real-time monitoring of anti-factor Xa level had been explored using an integrated microdialysis-coupled microfluidic system. By customizing the dialysis membrane and utilizing a fluorogenic substrate, the performance of the integrated device was characterized and tested b...

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Main Authors:	Xingbei Zhou, Tao Ding, Shushui Wang, Longping Wen
Format:	Article
Language:	English
Published:	Elsevier 2021-12-01
Series:	Talanta Open
Subjects:	Anti-factor Xa Microdialysis Microfluidic Real-time monitoring Therapeutic drug monitoring
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666831921000291

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spelling	doaj-662bfa737457418d86271655d4c153d32021-09-11T04:31:27ZengElsevierTalanta Open2666-83192021-12-014100059In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic deviceXingbei Zhou0Tao Ding1Shushui Wang2Longping Wen3School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510006, China; Department of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510080, ChinaSchool of Medicine, South China University of Technology, Guangzhou, Guangdong, 510006, ChinaDepartment of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510080, China; Co-Corresponding author at: Department of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510080, China.School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510006, China; Nanobio Laboratory, Institute of Life Sciences, South China University of Technology, Guangzhou, Guangdong, 510006, China; Corresponding author at: School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.The feasibility of in vivo real-time monitoring of anti-factor Xa level had been explored using an integrated microdialysis-coupled microfluidic system. By customizing the dialysis membrane and utilizing a fluorogenic substrate, the performance of the integrated device was characterized and tested by continuous monitoring of the anti-factor Xa level in rats. Efficient sampling for low molecular weight heparin in the blood stream was demonstrated and the detection range of the device was determined to be 0.1-1.5 IU/ml. The anti-factor Xa profile was recorded for a period of 7 hours. Moreover, the results generated from this method agreed well with those from conventional assay. The present system provides a novel quantitative monitoring method with great precision, good reproducibility and full data coverage of treatment period. This study has paved the road for developing a bedside drug monitoring device in hospital setting.http://www.sciencedirect.com/science/article/pii/S2666831921000291Anti-factor XaMicrodialysisMicrofluidicReal-time monitoringTherapeutic drug monitoring
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xingbei Zhou Tao Ding Shushui Wang Longping Wen
spellingShingle	Xingbei Zhou Tao Ding Shushui Wang Longping Wen In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device Talanta Open Anti-factor Xa Microdialysis Microfluidic Real-time monitoring Therapeutic drug monitoring
author_facet	Xingbei Zhou Tao Ding Shushui Wang Longping Wen
author_sort	Xingbei Zhou
title	In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device
title_short	In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device
title_full	In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device
title_fullStr	In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device
title_full_unstemmed	In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device
title_sort	in vivo real-time monitoring of anti-factor xa level using a microdialysis-coupled microfluidic device
publisher	Elsevier
series	Talanta Open
issn	2666-8319
publishDate	2021-12-01
description	The feasibility of in vivo real-time monitoring of anti-factor Xa level had been explored using an integrated microdialysis-coupled microfluidic system. By customizing the dialysis membrane and utilizing a fluorogenic substrate, the performance of the integrated device was characterized and tested by continuous monitoring of the anti-factor Xa level in rats. Efficient sampling for low molecular weight heparin in the blood stream was demonstrated and the detection range of the device was determined to be 0.1-1.5 IU/ml. The anti-factor Xa profile was recorded for a period of 7 hours. Moreover, the results generated from this method agreed well with those from conventional assay. The present system provides a novel quantitative monitoring method with great precision, good reproducibility and full data coverage of treatment period. This study has paved the road for developing a bedside drug monitoring device in hospital setting.
topic	Anti-factor Xa Microdialysis Microfluidic Real-time monitoring Therapeutic drug monitoring
url	http://www.sciencedirect.com/science/article/pii/S2666831921000291
work_keys_str_mv	AT xingbeizhou invivorealtimemonitoringofantifactorxalevelusingamicrodialysiscoupledmicrofluidicdevice AT taoding invivorealtimemonitoringofantifactorxalevelusingamicrodialysiscoupledmicrofluidicdevice AT shushuiwang invivorealtimemonitoringofantifactorxalevelusingamicrodialysiscoupledmicrofluidicdevice AT longpingwen invivorealtimemonitoringofantifactorxalevelusingamicrodialysiscoupledmicrofluidicdevice
_version_	1717757057023606784

In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device

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