| Summary: | Abstract Background The freeze-thaw process results in structural and functional damages caused by over accumulation of reactive oxygen species (ROS). Addition of exogenous antioxidants to semen extender is of a great importance to overcome the oxidative damage during the freezing process. The present study aimed to clarify the impact of Diospyros kaki on preserved buffalo semen quality. Tris extender enhanced with kaki juice (KEE) at concentrations 0% (control) and 1-10% (v/v). A twenty percent of freshly prepared egg yolk was added to the KEE extender (KEEY), then all tubes were centrifuged to eliminate any debris. Semen was added to the supernatants in other tubes. Semen was evaluated and the conception rate was implemented. Results Sperm forward motility was significantly (P < 0.0001) kept high after 5 days of chilling for the concentrations 3, 4, and 5% with respect to the control and kept high (P < 0.01) at the other concentrations till the 4th day of chilling. Addition of KEE had significantly (P < 0.03) ameliorated post freezing sperm motility with all the concentrations of the extender except the concentration (10%). The highest spermatozoal motility was obtained with the concentration of 1% with respect to the control. Alive sperm%, abnormalities%, and % of intact spermatozoa membranes (HOST%) were similar to the control with all concentrations of kaki used. The conception rate was higher when 1-6% KEE were used. Conclusions Some concentrations of Diospyros kaki improved buffalo bull semen quality post-chilling, post-freezing, the conception rate, and the concentration 1% gave the best results.
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