The Changes in Status of Antioxidant Defence System on Temporary Cerebral Ischemia and the Protective Effect of Vitamin E and C

• Main Authors: Basra Obay Deniz, Abdurrahman Şermet, Cemil Tümer, Yüksel Koçyiğit, Nebahat Taşdemir, Mukadder Atmaca, Mustafa Kelle
• Format: Article
• Language: English
• Published: Dicle University Medical School 2004-01-01
• Series: Dicle Medical Journal
• Subjects: Cerebral Ischemia; Antioxidant Defence System; Lipid Peroxidation
• Online Access: http://4181.indexcopernicus.com/fulltxt.php?ICID=887945
• ISSN: 1300-2945; 1308-9889

Time-dependent changes in antioxidant defence system and the effect ofvitamin C and E supplementation on temporary cerebral ischemia wasinvestigated in this study. This study was performed in 140 male Wistaralbino rats weighting 300-400g. Before 30 minutes cerebral ischemiavitamin E (α-tocopherol) 10 mg/kg/day and vitamin C 30 mg/kg/day wereapplied intraperitoneally for a week period. Animals were sacrifiedfollowing 24 h and 72 h ischemia and the brain was removed as quick aspossible. Brain water content, süperoxide dismutase (SOD) ) and catalase(CAT) activities and the level of nonenzymatic antioxidant, reduced vitaminC and α-tocopherol levels were measured. Moreover the level ofmalondialdehyde (MDA) as an indicator of lipid peroxidation wasdetermined. At 24 h of reperfusion superoxide dismutase (SOD) andcatalase (CAT) activities, vitamin C and E level decreased significantly inischemic brain area (p<0.05, p<0.05, p<0.01 and p<0.01 respectively). After72 h of reperfusion SOD and CAT activities surpassed the pre-ischemiclevel (p<0.01 and p<0.05 respectively). The level of vitamin C and Eincreased significantly at 72 hours after ischemia but, did not reach preischemiclevels (p<0.01 and p<0.01 respectively). Administration of vitaminC and E a week before cerebral ischemia prevented the decrease in SODand CAT activities after 24 hours of reperfusion (p<0.01 and p<0.01respectively). Furthermore, the increase in ischemia induced lipidperoxidation and cerebral edema were decreased significantly byadministration of these vitamins (p<0.01 and p<0.01 respectively). Invitamins applied groups, increase in SOD and catalase activities at 72 hoursafter ischemia was similar to control. The results indicated that there isnegative correlation between vitamin C or E level and lipid peroxidation orcerebral edema. Similar relationship was found between SOD or CATactivitiy and lipid peroxidation or cerebral edama.Temporary cerebral ischemia changed the state of anti-oxidant defencesystem and these changes were significantly prevented by treatment with Cand E vitamins before ischemia.