Barcode-free next-generation sequencing error validation for ultra-rare variant detection
Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-08941-4 |