Barcode-free next-generation sequencing error validation for ultra-rare variant detection

Barcode-free next-generation sequencing error validation for ultra-rare variant detection

Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.

Bibliographic Details
Main Authors: Huiran Yeom, Yonghee Lee, Taehoon Ryu, Jinsung Noh, Amos Chungwon Lee, Han-Byoel Lee, Eunji Kang, Seo Woo Song, Sunghoon Kwon
Format: Article
Language:English
Published: Nature Publishing Group 2019-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-08941-4
Description
Summary:Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.
ISSN:2041-1723

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