| Summary: | In viticulture, detailed spatial information about actual evapotranspiration (ET<sub>a</sub>) and vine water status within a vineyard may be of particular utility when applying site-specific, precision irrigation management. Over recent decades, extensive research has been carried out in the use of remote sensing energy balance models to estimate and monitor ET<sub>a</sub> at the field level. However, one of the major limitations remains the coarse spatial resolution in the thermal infrared (TIR) domain. In this context, the recent advent of the Sentinel missions of the European Space Agency (ESA) has greatly improved the possibility of monitoring crop parameters and estimating ET<sub>a</sub> at higher temporal and spatial resolutions. In order to bridge the gap between the coarse-resolution Sentinel-3 thermal and the fine-resolution Sentinel-2 shortwave data, sharpening techniques have been used to downscale the Sentinel-3 land surface temperature (LST) from 1 km to 20 m. However, the accurate estimates of high-resolution LST through sharpening techniques are still unclear, particularly when intended to be used for detecting crop water stress. The goal of this study was to assess the feasibility of the two-source energy balance model (TSEB) using sharpened LST images from Sentinel-2 and Sentinel-3 (TSEB-PT<sub>S2+3</sub>) to estimate the spatio-temporal variability of actual transpiration (T) and water stress in a vineyard. T and crop water stress index (CWSI) estimates were evaluated against a vine water consumption model and regressed with in situ stem water potential (Y<sub>stem</sub>). Two different TSEB approaches, using very high-resolution airborne thermal imagery, were also included in the analysis as benchmarks for TSEB-PT<sub>S2+3</sub>. One of them uses aggregated TIR data at the vine+inter-row level (TSEB-PT<sub>airb</sub>), while the other is based on a contextual method that directly, although separately, retrieves soil and canopy temperatures (TSEB-2T). The results obtained demonstrated that when comparing airborne T<sub>rad</sub> and sharpened S2+3 LST, the latter tend to be underestimated. This complicates the use of TSEB-PT<sub>S2+3 </sub>to detect crop water stress. TSEB-2T appeared to outperform all the other methods. This was shown by a higher <i>R</i><sup>2</sup> and slightly lower RMSD when compared with modelled T. In addition, regressions between T and CWSI-2T with Y<sub>stem</sub> also produced the highest <i>R</i><sup>2</sup>.
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