Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods

About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endos...

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Main Authors:	R. Bacsó, G. Facsar, F. Gyulai, Gy. D. Bisztray, I. Velich
Format:	Article
Language:	English
Published:	University of Debrecen 2004-11-01
Series:	International Journal of Horticultural Science
Subjects:	mediaeval seeds fruits comel grape RAPD-PCR
Online Access:	https://ojs.lib.unideb.hu/IJHS/article/view/522

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spelling	doaj-6577e8eac3b44ac982fed4d71e556f052020-11-25T02:58:23ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X2004-11-0110410.31421/IJHS/10/4/522Examinations of 600-year-old seeds by means of archaeobotanical and genetical methodsR. Bacsó0G. Facsar1F. Gyulai2Gy. D. Bisztray3I. Velich4Corvinus University of Budapest, Faculty of Horticultural Sciences, Department of Genetics and Horticultural Plant Breeding, Budapest, HungaryCorvinus University of Budapest, Faculty of Horticultural Sciences, Department of Botany, Budapest, HungaryAgrobotanical Institute, Tápiószele, HungaryCorvinus University of Budapest, Faculty of Horticultural Sciences, Department of Genetics and Horticultural Plant Breeding, Budapest, HungaryCorvinus University of Budapest, Faculty of Horticultural Sciences, Department of Genetics and Horticultural Plant Breeding, Budapest, Hungary About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible. https://ojs.lib.unideb.hu/IJHS/article/view/522mediaeval seedsfruitscomelgrapeRAPD-PCR
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	R. Bacsó G. Facsar F. Gyulai Gy. D. Bisztray I. Velich
spellingShingle	R. Bacsó G. Facsar F. Gyulai Gy. D. Bisztray I. Velich Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods International Journal of Horticultural Science mediaeval seeds fruits comel grape RAPD-PCR
author_facet	R. Bacsó G. Facsar F. Gyulai Gy. D. Bisztray I. Velich
author_sort	R. Bacsó
title	Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
title_short	Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
title_full	Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
title_fullStr	Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
title_full_unstemmed	Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
title_sort	examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
publisher	University of Debrecen
series	International Journal of Horticultural Science
issn	1585-0404 2676-931X
publishDate	2004-11-01
description	About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible.
topic	mediaeval seeds fruits comel grape RAPD-PCR
url	https://ojs.lib.unideb.hu/IJHS/article/view/522
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Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods

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