Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods

About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endos...

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Bibliographic Details
Main Authors: R. Bacsó, G. Facsar, F. Gyulai, Gy. D. Bisztray, I. Velich
Format: Article
Language:English
Published: University of Debrecen 2004-11-01
Series:International Journal of Horticultural Science
Subjects:
Online Access:https://ojs.lib.unideb.hu/IJHS/article/view/522
Description
Summary:About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible.
ISSN:1585-0404
2676-931X