Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells

Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells

Dry-cured hams are well-known and highly appreciated products in the Mediterranean and China. The long-term fermentation endows dry-cured hams with a unique flavor and quality. Our previous study has identified Asp–Leu–Glu–Glu (DLEE) from dry-cured Xuanwei ham with remarkable antioxidant capacity. I...

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Main Authors: Lujuan Xing, Lijuan Fu, Yuejing Hao, Wangang Zhang
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Antioxidants
Subjects:
bioactive peptide
dry-cured ham
Asp–Leu–Glu–Glu
antioxidant enzymes
Nfr2/Keap1
Online Access:https://www.mdpi.com/2076-3921/10/9/1354
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spelling doaj-6571aa12843b495f9e40dab29480fb142021-09-25T23:37:58ZengMDPI AGAntioxidants2076-39212021-08-01101354135410.3390/antiox10091354Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 CellsLujuan Xing0Lijuan Fu1Yuejing Hao2Wangang Zhang3Key Lab of Meat Processing and Quality Control, Jiangsu Collaborative Innovation Center of Meat Production and Processing, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Lab of Meat Processing and Quality Control, Jiangsu Collaborative Innovation Center of Meat Production and Processing, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Lab of Meat Processing and Quality Control, Jiangsu Collaborative Innovation Center of Meat Production and Processing, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Lab of Meat Processing and Quality Control, Jiangsu Collaborative Innovation Center of Meat Production and Processing, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDry-cured hams are well-known and highly appreciated products in the Mediterranean and China. The long-term fermentation endows dry-cured hams with a unique flavor and quality. Our previous study has identified Asp–Leu–Glu–Glu (DLEE) from dry-cured Xuanwei ham with remarkable antioxidant capacity. In the current study, the Caco-2 cells were cultured in vitro and treated with different doses of DLEE. The cellular reactive oxygen species (ROS) level and antioxidant enzyme activities were then determined to investigate the intracellular protection effect of DLEE. According to the results, the cellular ROS level was reduced, whereas the antioxidant enzyme activities of glutathione reductase, catalase, and glutathione peroxidase were improved following DLEE treatment. The DLEE treatment also increased the Nrf2 expression, along with downregulating the Keap1 expression. Thus, the dry-cured ham-derived peptide DLEE exhibited excellent bioactive capacity by reducing the ROS level and regulating the antioxidant enzyme activities. In addition, Nrf2/Keap1 was shown to be the main signaling pathway underlying DLEE-induced antioxidant activities in Caco-2 cells.https://www.mdpi.com/2076-3921/10/9/1354bioactive peptidedry-cured hamAsp–Leu–Glu–Gluantioxidant enzymesNfr2/Keap1
collection DOAJ
language English
format Article
sources DOAJ
author Lujuan Xing
Lijuan Fu
Yuejing Hao
Wangang Zhang
spellingShingle Lujuan Xing
Lijuan Fu
Yuejing Hao
Wangang Zhang
Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells
Antioxidants
bioactive peptide
dry-cured ham
Asp–Leu–Glu–Glu
antioxidant enzymes
Nfr2/Keap1
author_facet Lujuan Xing
Lijuan Fu
Yuejing Hao
Wangang Zhang
author_sort Lujuan Xing
title Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells
title_short Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells
title_full Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells
title_fullStr Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells
title_full_unstemmed Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells
title_sort dry-cured ham-derived peptide (asp–leu–glu–glu) exerts cytoprotective capacity in human intestinal epithelial caco-2 cells
publisher MDPI AG
series Antioxidants
issn 2076-3921
publishDate 2021-08-01
description Dry-cured hams are well-known and highly appreciated products in the Mediterranean and China. The long-term fermentation endows dry-cured hams with a unique flavor and quality. Our previous study has identified Asp–Leu–Glu–Glu (DLEE) from dry-cured Xuanwei ham with remarkable antioxidant capacity. In the current study, the Caco-2 cells were cultured in vitro and treated with different doses of DLEE. The cellular reactive oxygen species (ROS) level and antioxidant enzyme activities were then determined to investigate the intracellular protection effect of DLEE. According to the results, the cellular ROS level was reduced, whereas the antioxidant enzyme activities of glutathione reductase, catalase, and glutathione peroxidase were improved following DLEE treatment. The DLEE treatment also increased the Nrf2 expression, along with downregulating the Keap1 expression. Thus, the dry-cured ham-derived peptide DLEE exhibited excellent bioactive capacity by reducing the ROS level and regulating the antioxidant enzyme activities. In addition, Nrf2/Keap1 was shown to be the main signaling pathway underlying DLEE-induced antioxidant activities in Caco-2 cells.
topic bioactive peptide
dry-cured ham
Asp–Leu–Glu–Glu
antioxidant enzymes
Nfr2/Keap1
url https://www.mdpi.com/2076-3921/10/9/1354
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AT yuejinghao drycuredhamderivedpeptideaspleuglugluexertscytoprotectivecapacityinhumanintestinalepithelialcaco2cells
AT wangangzhang drycuredhamderivedpeptideaspleuglugluexertscytoprotectivecapacityinhumanintestinalepithelialcaco2cells
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