Enzymological determination of free carnitine concentrations in rat tissues
Purified carnitine acetyltransferase was employed to catalyze the reaction:Carnitine+acetylCoA⇌acetylcarnitine+CoA. In the presence of a known excess of acetyl CoA, free CoA released was determined simultaneously in a coupled spectrophotometric assay. CoA formed was shown to be directly related to c...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1964-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520402366 |
| Summary: | Purified carnitine acetyltransferase was employed to catalyze the reaction:Carnitine+acetylCoA⇌acetylcarnitine+CoA. In the presence of a known excess of acetyl CoA, free CoA released was determined simultaneously in a coupled spectrophotometric assay. CoA formed was shown to be directly related to carnitine initially present. Rat tissue extracts were analyzed using this method, and μmoles of (–)-carnitine per gram dry weight were found to be 4.87 for heart; 3.14 for brown adipose tissue; 2.68 for skeletal muscle; 1.74 for testes; 1.26 for kidney; 0.55 for brain; and 0.051 for serum. The specificity and accuracy of the determinations are described. |
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| ISSN: | 0022-2275 |