Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling

Chronic obstructive pulmonary disease (COPD) is associated with inflammatory cell reactions, tissue destruction and lung remodeling. Many signaling pathways for these phenomena are still to be identified. We developed a mouse model of COPD to evaluate some pathophysiological mechanisms acting during...

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Main Authors: P.J.C. Biselli, F.D.T.Q.S. Lopes, H.T. Moriya, D.H.R.F. Rivero, A.C. Toledo, P.H.N. Saldiva, T. Mauad, M.A. Martins
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2011-05-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500012
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spelling doaj-64f7c45d6fca44d38310376ccaec2da72020-11-24T22:24:32ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2011-05-01445460468Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodelingP.J.C. BiselliF.D.T.Q.S. LopesH.T. MoriyaD.H.R.F. RiveroA.C. ToledoP.H.N. SaldivaT. MauadM.A. MartinsChronic obstructive pulmonary disease (COPD) is associated with inflammatory cell reactions, tissue destruction and lung remodeling. Many signaling pathways for these phenomena are still to be identified. We developed a mouse model of COPD to evaluate some pathophysiological mechanisms acting during the initial stage of the disease. Forty-seven 6- to 8-week-old female C57/BL6 mice (approximately 22 g) were exposed for 2 months to cigarette smoke and/or residual oil fly ash (ROFA), a concentrate of air pollution. We measured lung mechanics, airspace enlargement, airway wall thickness, epithelial cell profile, elastic and collagen fiber deposition, and by immunohistochemistry transforming growth factor-β1 (TGF-β1), macrophage elastase (MMP12), neutrophils and macrophages. We observed regional airspace enlargements near terminal bronchioles associated with the exposure to smoke or ROFA. There were also increases in airway resistance and thickening of airway walls in animals exposed to smoke. In the epithelium, we noted a decrease in the ciliated cell area of animals exposed to smoke and an increase in the total cell area associated with exposure to both smoke and ROFA. There was also an increase in the expression of TGF-β1 both in the airways and parenchyma of animals exposed to smoke. However, we could not detect inflammatory cell recruitment, increases in MMP12 or elastic and collagen fiber deposition. After 2 months of exposure to cigarette smoke and/or ROFA, mice developed regional airspace enlargements and airway epithelium remodeling, although no inflammation or increases in fiber deposition were detected. Some of these phenomena may have been mediated by TGF-β1.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500012EmphysemaCigarette smokeAirwaysTGF-β1Mouse airway epithelium remodelingProximal airspace enlargements
collection DOAJ
language English
format Article
sources DOAJ
author P.J.C. Biselli
F.D.T.Q.S. Lopes
H.T. Moriya
D.H.R.F. Rivero
A.C. Toledo
P.H.N. Saldiva
T. Mauad
M.A. Martins
spellingShingle P.J.C. Biselli
F.D.T.Q.S. Lopes
H.T. Moriya
D.H.R.F. Rivero
A.C. Toledo
P.H.N. Saldiva
T. Mauad
M.A. Martins
Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
Brazilian Journal of Medical and Biological Research
Emphysema
Cigarette smoke
Airways
TGF-β1
Mouse airway epithelium remodeling
Proximal airspace enlargements
author_facet P.J.C. Biselli
F.D.T.Q.S. Lopes
H.T. Moriya
D.H.R.F. Rivero
A.C. Toledo
P.H.N. Saldiva
T. Mauad
M.A. Martins
author_sort P.J.C. Biselli
title Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
title_short Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
title_full Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
title_fullStr Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
title_full_unstemmed Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
title_sort short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling
publisher Associação Brasileira de Divulgação Científica
series Brazilian Journal of Medical and Biological Research
issn 0100-879X
1414-431X
publishDate 2011-05-01
description Chronic obstructive pulmonary disease (COPD) is associated with inflammatory cell reactions, tissue destruction and lung remodeling. Many signaling pathways for these phenomena are still to be identified. We developed a mouse model of COPD to evaluate some pathophysiological mechanisms acting during the initial stage of the disease. Forty-seven 6- to 8-week-old female C57/BL6 mice (approximately 22 g) were exposed for 2 months to cigarette smoke and/or residual oil fly ash (ROFA), a concentrate of air pollution. We measured lung mechanics, airspace enlargement, airway wall thickness, epithelial cell profile, elastic and collagen fiber deposition, and by immunohistochemistry transforming growth factor-β1 (TGF-β1), macrophage elastase (MMP12), neutrophils and macrophages. We observed regional airspace enlargements near terminal bronchioles associated with the exposure to smoke or ROFA. There were also increases in airway resistance and thickening of airway walls in animals exposed to smoke. In the epithelium, we noted a decrease in the ciliated cell area of animals exposed to smoke and an increase in the total cell area associated with exposure to both smoke and ROFA. There was also an increase in the expression of TGF-β1 both in the airways and parenchyma of animals exposed to smoke. However, we could not detect inflammatory cell recruitment, increases in MMP12 or elastic and collagen fiber deposition. After 2 months of exposure to cigarette smoke and/or ROFA, mice developed regional airspace enlargements and airway epithelium remodeling, although no inflammation or increases in fiber deposition were detected. Some of these phenomena may have been mediated by TGF-β1.
topic Emphysema
Cigarette smoke
Airways
TGF-β1
Mouse airway epithelium remodeling
Proximal airspace enlargements
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500012
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