Drought Stress Tolerance and Photosynthetic Activity of Alloplasmic Lines <i>T. dicoccum</i> x <i>T. aestivum</i>

• Main Authors: Nina V. Terletskaya, Andrey B. Shcherban, Michail A. Nesterov, Roman N. Perfil’ev, Elena A. Salina, Nazira A. Altayeva, Irina V. Blavachinskaya
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: International Journal of Molecular Sciences
• Subjects: alloplasmic wheat lines; drought tolerance; photosynthesis; <i>DREB</i>; <i>orf256</i>; <i>rps19-p</i> genes
• Online Access: https://www.mdpi.com/1422-0067/21/9/3356
• ISSN: 1661-6596; 1422-0067

Tetraploid species <i>T. dicoccum</i> Shuebl is a potential source of drought tolerance for cultivated wheat, including common wheat. This paper describes the genotyping of nine stable allolines isolated in the offspring from crossing of <i>T. dicoccum</i> x <i>T. aestivum</i> L. using 21 microsatellite (simple sequence repeats—SSR) markers and two cytoplasmic mitochondrial markers to orf256, rps19-p genes; evaluation of drought tolerance of allolines at different stages of ontogenesis (growth parameters, relative water content, quantum efficiency of Photosystem II, electron transport rate, energy dissipated in Photosystem II); and the study of drought tolerance regulator gene <i>Dreb-1</i> with allele-specific PCR (AS-MARKER) and partial sequence analysis. Most allolines differ in genomic composition and <i>T. dicoccum</i> introgressions. Four allolines—D-b-05, D-d-05, D-d-05b, and D-41-05—revealed signs of drought tolerance of varying degrees. The more drought tolerant D-41-05 line was also characterized by <i>Dreb-B1</i> allele introgression from <i>T. dicoccum</i>. A number of non-specific patterns and significant differences in allolines in regulation of physiological parameters in drought conditions is identified. Changes in photosynthetic activity in stress-drought are shown to reflect the level of drought tolerance of the forms studied. The contribution of different combinations of nuclear/cytoplasmic genome and alleles of <i>Dreb-1 gene</i> in allolines to the formation of stress tolerance and photosynthetic activity is discussed.