A PROTOCOL FOR SHOOT REGENERATION FROM LEAVES PETIOLES TISSUE CULTURE OF NEEM TREES ( Melia azedarach )
Shoots were regenerated from petioles – derived callus on solidified Murashige and Skoog (MS) medium supplemented with 1.5 mg / l BA only and MS medium containing 4.0 mg / l IBA and 1.0 mg / l BA . The percentage of shoot formation was 30.0 % . The regeneration frequency was reasonable to obtained...
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| Format: | Article |
| Language: | Arabic |
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College of Agriculture
2006-03-01
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| Series: | Mesopotamia Journal of Agriculture |
| Online Access: | https://magrj.mosuljournals.com/article_38498_e5ea1db26df8e8f644d24235f4bf9241.pdf |
| Summary: | Shoots were regenerated from petioles – derived callus on solidified Murashige and Skoog (MS) medium supplemented with 1.5 mg / l BA only and MS medium containing 4.0 mg / l IBA and 1.0 mg / l BA . The percentage of shoot formation was 30.0 % . The regeneration frequency was reasonable to obtained sufficient shoots by this method . This simple protocol is the first report in tissue culture of this woody plant which encourage other researchers to worke other studies in tissue culture of neem . Moreover , callus culture will be useful to obtain a wide range of industrial plant products . |
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| ISSN: | 1815-316X 2224-9796 |