Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process

• Main Authors: Ranjan Singh, Rajeeva Gaur, Soni Tiwari, Manogya Kumar Gaur
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Microbiologia 2012-09-01
• Series: Brazilian Journal of Microbiology
• Subjects: Aureobasidium pullulans; Pullulan; Non-stirred fed-batch fermentation
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300030

Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.