| Summary: | 【Abstract】Objective: To observe the effect of recombinant interleukin-6 (IL-6) and osteoprotegerin (OPG) on inhibiting bone absorption induced by receptor activa-
tor for nuclear factor-κB ligand (RANKL) in murine osteo-
clast precursor cells (OCPs) model.
Methods: RAW 264.7 cells were solely treated with 50 ng/ml RANKL for 1 day, and then they were divided into three groups: RANKL (control group), RANKL+IL-6 (IL-6
group) and RANKL+IL-6+OPG (combination group). These cells were harvested and investigated by means of HE stain-
ing under light microscope after consecutive 9 days. Furthermore, staining tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells were detected by inverted phase contrast microscope. The absorption pits of
bone slices were observed under scanning electron
microscope.
Results: The number of mature osteoclast cells in
control group was more than that in IL-6 alone or IL-6 com-
bined with OPG group (P<0.05). Interestingly, this experi-
ment has also demonstrated that there was a large number of TRAP-positive multinucleated osteoclasts (more than 3
nuclei) and several bone absorption formation in the con-
trol group, whereas the outcome was completely different
in both IL-6 group and IL-6+OPG group (P<0.05).
Conclusion: IL-6 can suppress the differentiation of
mature osteoclasts as directly adding it into the RAW 264.7
cells induced by 50 ng/ml RANKL, and further the effect of
osteolysis is remarkably reduced. When treatment with IL-
6 combined with OPG, a more effective strategy for the treat-
ment of osteoporosis is reached.
Key words: Osteoclasts; Osteoporosis; Osteopro-
tegerin; RANK ligand; Interleukin-6
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