<b>Extraction and anticoagulant activity of sulfated polysaccharides from <em>Caulerpa cupressoides</em> var. <em>lycopodium</em> (Vahl) C. Agardh (Chlorophyceae)</b> - doi: 10.4025/actascibiolsci.v33i2.6243 <b>Extraction and anticoagulant activity of sulfated polysaccharides from <em>Caulerpa cupressoides</em> var. <em>lycopodium</em> (Vahl) C. Agardh (Chlorophyceae)</b> - doi: 10.4025/actascibiolsci.v33i2.6243

• Main Authors: Norma Maria Barros Benevides, Ismael Nilo Lino de Queiroz, Bruno Pedrosa Fontes, Ana Luíza Gomes Quinderé, Edfranck de Sousa Oliveira Vanderlei, José Ariévilo Gurgel Rodrigues
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2011-05-01
• Series: Acta Scientiarum : Biological Sciences
• Subjects: Chlorophyta; sequential extractions; physical-chemical characterization; coagulation test
• Online Access: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6243

The reportedly low standard quality of heparin (HEP) for use in cardiac surgeries has led to concern in the Brazilian and international markets. Sulfated polysaccharides (SPs) from seaweeds have been regarded as promising substitutes for HEP. The aim of this study was to sequentially extract total SPs (TSPs) from <em>Caulerpa cupressoides</em> (Chlorophyceae) with papain in 100 mM sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by fractionation by ion-exchange chromatography (DEAE-cellulose), and then evaluate the anticoagulant potential of SP fractions by activated partial thromboplastin time (APTT) using normal human plasma and compare it to standard HEP (193 IU mg^-1). The obtained fractions were chemically characterized by chemical composition and agarose gel electrophoresis. The yield was 4.61%, and three fractions of SP (F I, F II and F III) eluted with 0.50, 0.75 and 1.00 M of NaCl, respectively, were observed on chromatography profiles; however, differences in charge densities patterns and degree of resolution among them were revealed by electrophoresis. SPs were capable of modifying APTT only in fractions eluted with 0.75 M of NaCl, whose activities were 23.37 and 25.76 IU mg^-1, respectively, and the charge density was prerequisite to activity. Therefore, C. <em>cupressoides</em> is a source of SPs possessing low anticoagulant potential compared to HEP.<br>The reportedly low standard quality of heparin (HEP) for use in cardiac surgeries has led to concern in the Brazilian and international markets. Sulfated polysaccharides (SPs) from seaweeds have been regarded as promising substitutes for HEP. The aim of this study was to sequentially extract total SPs (TSPs) from <em>Caulerpa cupressoides</em> (Chlorophyceae) with papain in 100 mM sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by fractionation by ion-exchange chromatography (DEAE-cellulose), and then evaluate the anticoagulant potential of SP fractions by activated partial thromboplastin time (APTT) using normal human plasma and compare it to standard HEP (193 IU mg^-1). The obtained fractions were chemically characterized by chemical composition and agarose gel electrophoresis. The yield was 4.61%, and three fractions of SP (F I, F II and F III) eluted with 0.50, 0.75 and 1.00 M of NaCl, respectively, were observed on chromatography profiles; however, differences in charge densities patterns and degree of resolution among them were revealed by electrophoresis. SPs were capable of modifying APTT only in fractions eluted with 0.75 M of NaCl, whose activities were 23.37 and 25.76 IU mg^-1, respectively, and the charge density was prerequisite to activity. Therefore, C. <em>cupressoides</em> is a source of SPs possessing low anticoagulant potential compared to HEP.