RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36

RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36

DHX36 is a G-quadruplex (G4) resolving helicase that targets both DNA-G4 and RNA-G4. Here the authors use single molecule FRET measurements and show that DHX36 resolves RNA-G4 structures by a mechanism involving an ATP-dependent, highly repetitive and stepwise refolding of RNA-G4 that differs from i...

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Main Authors: Ramreddy Tippana, Michael C. Chen, Natalia A. Demeshkina, Adrian R. Ferré-D’Amaré, Sua Myong
Format: Article
Language:English
Published: Nature Publishing Group 2019-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09802-w
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spelling doaj-647ccd90166c47d89791e381e1f5cdef2021-05-11T11:46:45ZengNature Publishing GroupNature Communications2041-17232019-04-0110111010.1038/s41467-019-09802-wRNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36Ramreddy Tippana0Michael C. Chen1Natalia A. Demeshkina2Adrian R. Ferré-D’Amaré3Sua Myong4Department of Biophysics, Johns Hopkins UniversityDepartment of Chemistry, University of CambridgeBiochemistry and Biophysics Center, National Heart, Lung and Blood InstituteBiochemistry and Biophysics Center, National Heart, Lung and Blood InstituteDepartment of Biophysics, Johns Hopkins UniversityDHX36 is a G-quadruplex (G4) resolving helicase that targets both DNA-G4 and RNA-G4. Here the authors use single molecule FRET measurements and show that DHX36 resolves RNA-G4 structures by a mechanism involving an ATP-dependent, highly repetitive and stepwise refolding of RNA-G4 that differs from its DNA-G4 structures resolving mechanism.https://doi.org/10.1038/s41467-019-09802-w
collection DOAJ
language English
format Article
sources DOAJ
author Ramreddy Tippana
Michael C. Chen
Natalia A. Demeshkina
Adrian R. Ferré-D’Amaré
Sua Myong
spellingShingle Ramreddy Tippana
Michael C. Chen
Natalia A. Demeshkina
Adrian R. Ferré-D’Amaré
Sua Myong
RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
Nature Communications
author_facet Ramreddy Tippana
Michael C. Chen
Natalia A. Demeshkina
Adrian R. Ferré-D’Amaré
Sua Myong
author_sort Ramreddy Tippana
title RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
title_short RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
title_full RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
title_fullStr RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
title_full_unstemmed RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36
title_sort rna g-quadruplex is resolved by repetitive and atp-dependent mechanism of dhx36
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-04-01
description DHX36 is a G-quadruplex (G4) resolving helicase that targets both DNA-G4 and RNA-G4. Here the authors use single molecule FRET measurements and show that DHX36 resolves RNA-G4 structures by a mechanism involving an ATP-dependent, highly repetitive and stepwise refolding of RNA-G4 that differs from its DNA-G4 structures resolving mechanism.
url https://doi.org/10.1038/s41467-019-09802-w
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AT nataliaademeshkina rnagquadruplexisresolvedbyrepetitiveandatpdependentmechanismofdhx36
AT adrianrferredamare rnagquadruplexisresolvedbyrepetitiveandatpdependentmechanismofdhx36
AT suamyong rnagquadruplexisresolvedbyrepetitiveandatpdependentmechanismofdhx36
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