The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density

The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density

Poplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the <i>PdERECTA</i> gene, which is a member of the LRR-RLKs family from <i>Populus nigra<...

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Main Authors: Huiguang Li, Yanli Yang, Houling Wang, Sha Liu, Fuli Jia, Yanyan Su, Shuang Li, Fang He, Conghua Feng, Mengxue Niu, Jie Wang, Chao Liu, Weilun Yin, Xinli Xia
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Populus</i>
<i>ERECTA</i>
stomatal density
water use efficiency
drought stress tolerance
Online Access:https://www.mdpi.com/1422-0067/22/14/7245
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language English
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author Huiguang Li
Yanli Yang
Houling Wang
Sha Liu
Fuli Jia
Yanyan Su
Shuang Li
Fang He
Conghua Feng
Mengxue Niu
Jie Wang
Chao Liu
Weilun Yin
Xinli Xia
spellingShingle Huiguang Li
Yanli Yang
Houling Wang
Sha Liu
Fuli Jia
Yanyan Su
Shuang Li
Fang He
Conghua Feng
Mengxue Niu
Jie Wang
Chao Liu
Weilun Yin
Xinli Xia
The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
International Journal of Molecular Sciences
<i>Populus</i>
<i>ERECTA</i>
stomatal density
water use efficiency
drought stress tolerance
author_facet Huiguang Li
Yanli Yang
Houling Wang
Sha Liu
Fuli Jia
Yanyan Su
Shuang Li
Fang He
Conghua Feng
Mengxue Niu
Jie Wang
Chao Liu
Weilun Yin
Xinli Xia
author_sort Huiguang Li
title The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
title_short The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
title_full The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
title_fullStr The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
title_full_unstemmed The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
title_sort receptor-like kinase <i>erecta</i> confers improved water use efficiency and drought tolerance to poplar via modulating stomatal density
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2021-07-01
description Poplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the <i>PdERECTA</i> gene, which is a member of the LRR-RLKs family from <i>Populus nigra</i> × (<i>Populus deltoides</i> × <i>Populus nigra</i>), improves water use efficiency and enhances drought tolerance in triploid white poplar. <i>PdERECTA</i> localizes to the plasma membrane. Overexpression plants showed lower stomatal density and larger stomatal size. The abaxial stomatal density was 24–34% lower and the stomatal size was 12–14% larger in overexpression lines. Reduced stomatal density led to a sharp restriction of transpiration, which was about 18–35% lower than the control line, and instantaneous water use efficiency was around 14–63% higher in overexpression lines under different conditions. These phenotypic changes led to increased drought tolerance. <i>PdERECTA</i> overexpression plants not only survived longer after stopping watering but also performed better when supplied with limited water, as they had better physical and photosynthesis conditions, faster growth rate, and higher biomass accumulation. Taken together, our data suggest that <i>PdERECTA</i> can alter the development pattern of stomata to reduce stomatal density, which then restricts water consumption, conferring enhanced drought tolerance to poplar. This makes <i>PdERECTA</i> trees promising candidates for establishing more water use efficient plantations.
topic <i>Populus</i>
<i>ERECTA</i>
stomatal density
water use efficiency
drought stress tolerance
url https://www.mdpi.com/1422-0067/22/14/7245
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spelling doaj-6470ba7a35244bd2a3c1b967f718e1e92021-07-23T13:45:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-01227245724510.3390/ijms22147245The Receptor-Like Kinase <i>ERECTA</i> Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal DensityHuiguang Li0Yanli Yang1Houling Wang2Sha Liu3Fuli Jia4Yanyan Su5Shuang Li6Fang He7Conghua Feng8Mengxue Niu9Jie Wang10Chao Liu11Weilun Yin12Xinli Xia13Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, ChinaPoplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the <i>PdERECTA</i> gene, which is a member of the LRR-RLKs family from <i>Populus nigra</i> × (<i>Populus deltoides</i> × <i>Populus nigra</i>), improves water use efficiency and enhances drought tolerance in triploid white poplar. <i>PdERECTA</i> localizes to the plasma membrane. Overexpression plants showed lower stomatal density and larger stomatal size. The abaxial stomatal density was 24–34% lower and the stomatal size was 12–14% larger in overexpression lines. Reduced stomatal density led to a sharp restriction of transpiration, which was about 18–35% lower than the control line, and instantaneous water use efficiency was around 14–63% higher in overexpression lines under different conditions. These phenotypic changes led to increased drought tolerance. <i>PdERECTA</i> overexpression plants not only survived longer after stopping watering but also performed better when supplied with limited water, as they had better physical and photosynthesis conditions, faster growth rate, and higher biomass accumulation. Taken together, our data suggest that <i>PdERECTA</i> can alter the development pattern of stomata to reduce stomatal density, which then restricts water consumption, conferring enhanced drought tolerance to poplar. This makes <i>PdERECTA</i> trees promising candidates for establishing more water use efficient plantations.https://www.mdpi.com/1422-0067/22/14/7245<i>Populus</i><i>ERECTA</i>stomatal densitywater use efficiencydrought stress tolerance

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