Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia

Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia

CTNNA3, first reported in association with arrhythmogenic right ventricular cardiomyopathy in 2003, is an unique component of both desmosomes and adherens junctions. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononucl...

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Main Authors: Cuilan Hou, Wei Liu, Lijian Xie, Junmin Zheng, Xiaonan Chen, Xiaomin Sun, Yongwei Zhang, Meng Xu, Yun Li, Tingting Xiao
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121000295
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spelling doaj-647048dec2c844f7bbd727a59e015e652021-03-13T04:22:17ZengElsevierStem Cell Research1873-50612021-03-0151102183Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmiaCuilan Hou0Wei Liu1Lijian Xie2Junmin Zheng3Xiaonan Chen4Xiaomin Sun5Yongwei Zhang6Meng Xu7Yun Li8Tingting Xiao9Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, ChinaDepartment of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China; Corresponding authors at: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai 200062, China.Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China; Corresponding authors at: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai 200062, China.CTNNA3, first reported in association with arrhythmogenic right ventricular cardiomyopathy in 2003, is an unique component of both desmosomes and adherens junctions. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells of a child with arrhythmia. The iPSCs exhibited stable amplification, expressed pluripotent markers, and differentiated spontaneously into three germ layers in vitro. Additionally, this iPSC line was found to maintain a normal karyotype and retain the pathogenic mutation in CTNNA3, facilitating a platform to study the disease mechanisms of arrhythmia and dysfunctions related to CTNNA3 mutations.http://www.sciencedirect.com/science/article/pii/S1873506121000295
collection DOAJ
language English
format Article
sources DOAJ
author Cuilan Hou
Wei Liu
Lijian Xie
Junmin Zheng
Xiaonan Chen
Xiaomin Sun
Yongwei Zhang
Meng Xu
Yun Li
Tingting Xiao
spellingShingle Cuilan Hou
Wei Liu
Lijian Xie
Junmin Zheng
Xiaonan Chen
Xiaomin Sun
Yongwei Zhang
Meng Xu
Yun Li
Tingting Xiao
Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia
Stem Cell Research
author_facet Cuilan Hou
Wei Liu
Lijian Xie
Junmin Zheng
Xiaonan Chen
Xiaomin Sun
Yongwei Zhang
Meng Xu
Yun Li
Tingting Xiao
author_sort Cuilan Hou
title Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia
title_short Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia
title_full Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia
title_fullStr Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia
title_full_unstemmed Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia
title_sort generation of an induced pluripotent stem cell line from a chinese han child with arrhythmia
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-03-01
description CTNNA3, first reported in association with arrhythmogenic right ventricular cardiomyopathy in 2003, is an unique component of both desmosomes and adherens junctions. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells of a child with arrhythmia. The iPSCs exhibited stable amplification, expressed pluripotent markers, and differentiated spontaneously into three germ layers in vitro. Additionally, this iPSC line was found to maintain a normal karyotype and retain the pathogenic mutation in CTNNA3, facilitating a platform to study the disease mechanisms of arrhythmia and dysfunctions related to CTNNA3 mutations.
url http://www.sciencedirect.com/science/article/pii/S1873506121000295
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