Use of constant composition polyvinylpyrrolidone columns to study the interaction of fat particles with plasma

• Main Authors: David D. O'Hara, Daniel Porte, Jr., Robert H. Williams
• Format: Article
• Language: English
• Published: Elsevier 1966-03-01
• Series: Journal of Lipid Research
• Subjects: dog; man; lymph; plasma; primary; secondary
• Online Access: http://www.sciencedirect.com/science/article/pii/S0022227520392919

Fat particles (lipoproteins of Sf > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v). As the concentration of PVP increased, a greater proportion of the particles accumulated at the top of the tube (``primary'' particles). When the concentration of PVP in the tube was held constant at 3% PVP, dilution of the sample with plasma was found to produce an increased proportion of bottom particles (``secondary'' particles). This observation suggests that bottom particles result from an interaction of top particles with plasma.