Use of constant composition polyvinylpyrrolidone columns to study the interaction of fat particles with plasma
Fat particles (lipoproteins of Sf > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v)....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1966-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520392919 |
| Summary: | Fat particles (lipoproteins of Sf > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v). As the concentration of PVP increased, a greater proportion of the particles accumulated at the top of the tube (``primary'' particles). When the concentration of PVP in the tube was held constant at 3% PVP, dilution of the sample with plasma was found to produce an increased proportion of bottom particles (``secondary'' particles). This observation suggests that bottom particles result from an interaction of top particles with plasma. |
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| ISSN: | 0022-2275 |