Association of rs1042658 Polymorphism of the CSF3 Gene with the Susceptibility of Recurrent Pregnancy Loss in Women of Fars Province

• Main Authors: Kobra Jahangirizade, Mahboobeh Nasiri, Naeimehossadat Asmarian
• Format: Article
• Language: fas
• Published: Arak Medical University 2016-04-01
• Series: Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk
• Subjects: CSF3; Cytokine; Polymorphism; Recurrent pregnancy loss
• Online Access: http://amuj.arakmu.ac.ir/article-1-3899-en.pdf

Abstract Background: Recurrent pregnancy loss (RPL) is a heterogeneous condition with the prevalence of more than 1% among women of reproductive age, which is defined as the occurrence of more than two miscarriages. Immune-mediators, cytokines, determine the role of immune cells in response to tissue incompatibility conditions. Colony stimulating factor 3 (CSF3) is a cytokine affecting the expression of other cytokines such as IL-4, and suppressing the immune response against semi-allograft embryo. This study was done for the first time on the association of the rs1042658 polymorphism at 3’UTR of the CSF3 gene with the susceptibility to recurrent pregnancy loss, supposing the effect of it on the expression level and stability of the CSF3 gene transcript. Materials and Methods: 122 RPL women and 140 healthy fertile women as a control group were enrolled in this case-control study. Genotype distribution of the selected polymorphism was evaluated by T-ARMS PCR method and the results were analyzed by logistic regression test. Results: comparison of the genotypic frequencies in the 3'UTR of the CSF3 gene in patients and controls resulted in the statistically significant difference in the incidence of pregnancy loss in order to the protection effect of the genotypes carrying T allele was observed between two groups (p<0.05). History of the abortion among the relatives of RPL women versus the relatives of controls showed significant differences (p=0.05). Conclusion: Findings showed significant relationship between rs1042658 polymorphism and the risk of recurrent pregnancy loss, which can affect the susceptibility of the condition.