APPROACHES TO ENGINEER STABILITY OF BEETLE LUCIFERASES

• Main Authors: Mikhail I. Koksharov, Natalia N. Ugarova
• Format: Article
• Language: English
• Published: Elsevier 2012-09-01
• Series: Computational and Structural Biotechnology Journal
• Subjects: Firefly luciferase; Bioluminescence; protein engineering; directed evolution; thermal stability
• Online Access: http://www.sciencedirect.com/science/article/pii/S2001037014600829

Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas. Many novel beetle luciferases with promising properties have been reported in the recent years. However, actual and potential applications of wild-type beetle luciferases are often limited by insufficient stability or decrease in activity of the enzyme at the conditions of a particular assay. Various examples of genetic engineering of the enhanced beetle luciferases have been reported that successfully solve or alleviate many of these limitations. This mini-review summarizes the recent advances in development of mutant luciferases with improved stability and activity characteristics. It discusses the common limitations of wild-type luciferases in different applications and presents the efficient approaches that can be used to address these problems.