A Recombinant Affinity Reagent Specific for a Phosphoepitope of Akt1

A Recombinant Affinity Reagent Specific for a Phosphoepitope of Akt1

The serine/threonine-protein kinase, Akt1, plays an important part in mammalian cell growth, proliferation, migration and angiogenesis, and becomes activated through phosphorylation. To monitor phosphorylation of threonine 308 in Akt1, we developed a recombinant phosphothreonine-binding domain (pTBD...

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Bibliographic Details
Main Authors: Jennifer E. McGinnis, Leon A. Venegas, Hector Lopez, Brian K. Kay
Format: Article
Language:English
Published: MDPI AG 2018-10-01
Series:International Journal of Molecular Sciences
Subjects:
affinity selection
alanine-scanning
Forkhead Associated (FHA) domain
phosphopeptide
phage-display
surface plasmon resonance (SPR)
Online Access:https://www.mdpi.com/1422-0067/19/11/3305

Internet

https://www.mdpi.com/1422-0067/19/11/3305

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