Extraction and Detection of Avian Influenza Virus From Wetland Sediment Using Enrichment-Based Targeted Resequencing

Extraction and Detection of Avian Influenza Virus From Wetland Sediment Using Enrichment-Based Targeted Resequencing

Early virus detection and characterization is key to successful avian influenza virus (AIV) surveillance for the health of humans as well as domestic poultry. We explored a novel sampling approach and molecular strategy using sediment from wetlands and outdoor waterbodies on poultry farms as a popul...

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Bibliographic Details
Main Authors: Lauren C. Tindale, Waren Baticados, Jun Duan, Michelle Coombe, Agatha Jassem, Patrick Tang, Miguel Uyaguari-Diaz, Richard Moore, Chelsea Himsworth, William Hsiao, Natalie Prystajecky
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Veterinary Science
Subjects:
avian influenza virus
next generation sequencing
nucleic acid extraction
RT-qPCR
surveillance
sediment
Online Access:https://www.frontiersin.org/article/10.3389/fvets.2020.00301/full

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