Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene

Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene

The KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plate...

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Main Authors: Laureen Jacquet, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000210
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spelling doaj-63b84af876314f1e81e45503871755dd2020-11-24T23:33:58ZengElsevierStem Cell Research1873-50611876-77532016-03-0116229329510.1016/j.scr.2016.01.011Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT geneLaureen JacquetHeema HewitsonVictoria WoodNeli KadevaGlenda CornwellStefano CodognottoEmma StephensonDusko IlicThe KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.http://www.sciencedirect.com/science/article/pii/S1873506116000210
collection DOAJ
language English
format Article
sources DOAJ
author Laureen Jacquet
Heema Hewitson
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Emma Stephenson
Dusko Ilic
spellingShingle Laureen Jacquet
Heema Hewitson
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Emma Stephenson
Dusko Ilic
Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
Stem Cell Research
author_facet Laureen Jacquet
Heema Hewitson
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Emma Stephenson
Dusko Ilic
author_sort Laureen Jacquet
title Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_short Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_full Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_fullStr Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_full_unstemmed Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_sort generation of kcl013 research grade human embryonic stem cell line carrying a mutation in the htt gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
url http://www.sciencedirect.com/science/article/pii/S1873506116000210
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AT victoriawood generationofkcl013researchgradehumanembryonicstemcelllinecarryingamutationinthehttgene
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