Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea

Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea

The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored t...

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Main Authors: Victor R. C. Merino, Viviane Nakano, Sydney M. Finegold, Mario J. Avila-Campos
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:Scientifica
Online Access:http://dx.doi.org/10.1155/2014/594014
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spelling doaj-6384c728745e472fb6c682d1ea116aee2020-11-25T02:09:18ZengHindawi LimitedScientifica2090-908X2014-01-01201410.1155/2014/594014594014Genes Encoding Toxin of Clostridium difficile in Children with and without DiarrheaVictor R. C. Merino0Viviane Nakano1Sydney M. Finegold2Mario J. Avila-Campos3Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, BrazilAnaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, BrazilVeterans Affairs, West Los Angeles Medical Center, Los Angeles, CA, USAAnaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, BrazilThe presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.http://dx.doi.org/10.1155/2014/594014
collection DOAJ
language English
format Article
sources DOAJ
author Victor R. C. Merino
Viviane Nakano
Sydney M. Finegold
Mario J. Avila-Campos
spellingShingle Victor R. C. Merino
Viviane Nakano
Sydney M. Finegold
Mario J. Avila-Campos
Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
Scientifica
author_facet Victor R. C. Merino
Viviane Nakano
Sydney M. Finegold
Mario J. Avila-Campos
author_sort Victor R. C. Merino
title Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_short Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_full Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_fullStr Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_full_unstemmed Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_sort genes encoding toxin of clostridium difficile in children with and without diarrhea
publisher Hindawi Limited
series Scientifica
issn 2090-908X
publishDate 2014-01-01
description The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.
url http://dx.doi.org/10.1155/2014/594014
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AT vivianenakano genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea
AT sydneymfinegold genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea
AT mariojavilacampos genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea
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