Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine

Peak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting in in vivo MR spectra. We constructed an in vitro model consistin...

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Main Authors: Karel Bernášek, Marián Grocký, Martin Burian, Jan Lang
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Journal of Spectroscopy
Online Access:http://dx.doi.org/10.1155/2016/4596542
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spelling doaj-634704f6c1fb4a788e0a4623fb61b4be2020-11-24T22:26:24ZengHindawi LimitedJournal of Spectroscopy2314-49202314-49392016-01-01201610.1155/2016/45965424596542Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of CarnosineKarel Bernášek0Marián Grocký1Martin Burian2Jan Lang3Department of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, 180 00 Prague, Czech RepublicDepartment of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, 180 00 Prague, Czech RepublicMR Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21 Prague, Czech RepublicDepartment of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, 180 00 Prague, Czech RepublicPeak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting in in vivo MR spectra. We constructed an in vitro model consisting of mechanically stretched gelatin cylinder soaked with the muscle metabolite carnosine. We describe the preparation procedure of an upscaled 50 mL stretched gelatin sample with carnosine that can be used as a phantom for setting-up and testing of spectroscopic measurements of RDC in a MR scanner. We also report on analysis of the RDC splittings in 1H and 13C high resolution MR spectra of carnosine.http://dx.doi.org/10.1155/2016/4596542
collection DOAJ
language English
format Article
sources DOAJ
author Karel Bernášek
Marián Grocký
Martin Burian
Jan Lang
spellingShingle Karel Bernášek
Marián Grocký
Martin Burian
Jan Lang
Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
Journal of Spectroscopy
author_facet Karel Bernášek
Marián Grocký
Martin Burian
Jan Lang
author_sort Karel Bernášek
title Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
title_short Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
title_full Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
title_fullStr Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
title_full_unstemmed Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
title_sort stretched gelatin phantom for detection of residual dipolar couplings in mr spectra and data analysis of carnosine
publisher Hindawi Limited
series Journal of Spectroscopy
issn 2314-4920
2314-4939
publishDate 2016-01-01
description Peak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting in in vivo MR spectra. We constructed an in vitro model consisting of mechanically stretched gelatin cylinder soaked with the muscle metabolite carnosine. We describe the preparation procedure of an upscaled 50 mL stretched gelatin sample with carnosine that can be used as a phantom for setting-up and testing of spectroscopic measurements of RDC in a MR scanner. We also report on analysis of the RDC splittings in 1H and 13C high resolution MR spectra of carnosine.
url http://dx.doi.org/10.1155/2016/4596542
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