Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine
Peak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting in in vivo MR spectra. We constructed an in vitro model consistin...
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Series: | Journal of Spectroscopy |
Online Access: | http://dx.doi.org/10.1155/2016/4596542 |
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doaj-634704f6c1fb4a788e0a4623fb61b4be2020-11-24T22:26:24ZengHindawi LimitedJournal of Spectroscopy2314-49202314-49392016-01-01201610.1155/2016/45965424596542Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of CarnosineKarel Bernášek0Marián Grocký1Martin Burian2Jan Lang3Department of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, 180 00 Prague, Czech RepublicDepartment of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, 180 00 Prague, Czech RepublicMR Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21 Prague, Czech RepublicDepartment of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, 180 00 Prague, Czech RepublicPeak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting in in vivo MR spectra. We constructed an in vitro model consisting of mechanically stretched gelatin cylinder soaked with the muscle metabolite carnosine. We describe the preparation procedure of an upscaled 50 mL stretched gelatin sample with carnosine that can be used as a phantom for setting-up and testing of spectroscopic measurements of RDC in a MR scanner. We also report on analysis of the RDC splittings in 1H and 13C high resolution MR spectra of carnosine.http://dx.doi.org/10.1155/2016/4596542 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karel Bernášek Marián Grocký Martin Burian Jan Lang |
spellingShingle |
Karel Bernášek Marián Grocký Martin Burian Jan Lang Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine Journal of Spectroscopy |
author_facet |
Karel Bernášek Marián Grocký Martin Burian Jan Lang |
author_sort |
Karel Bernášek |
title |
Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine |
title_short |
Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine |
title_full |
Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine |
title_fullStr |
Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine |
title_full_unstemmed |
Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine |
title_sort |
stretched gelatin phantom for detection of residual dipolar couplings in mr spectra and data analysis of carnosine |
publisher |
Hindawi Limited |
series |
Journal of Spectroscopy |
issn |
2314-4920 2314-4939 |
publishDate |
2016-01-01 |
description |
Peak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting in in vivo MR spectra. We constructed an in vitro model consisting of mechanically stretched gelatin cylinder soaked with the muscle metabolite carnosine. We describe the preparation procedure of an upscaled 50 mL stretched gelatin sample with carnosine that can be used as a phantom for setting-up and testing of spectroscopic measurements of RDC in a MR scanner. We also report on analysis of the RDC splittings in 1H and 13C high resolution MR spectra of carnosine. |
url |
http://dx.doi.org/10.1155/2016/4596542 |
work_keys_str_mv |
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