Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts.
BACKGROUND:The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain seg...
Main Authors: | Renata de Cássia-Pires, Myllena de Fátima Alheiros Dias de Melo, Raquel da Hora Barbosa, André Luiz Rodrigues Roque |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5354409?pdf=render |
Similar Items
-
KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
by: Janaína Sousa Campos Alvarenga, et al.
Published: (2012-01-01) -
GAPDH spike RNA as an alternative for housekeeping genes in relative gene expression assay using real-time PCR
by: Zeenah Weheed Atwan
Published: (2020-02-01) -
An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
by: Octavio Fernandes, et al.
Published: (1996-06-01) -
The effect of bisphosphonates on gene expression: GAPDH as a housekeeping or a new target gene?
by: Zanatta Mirko, et al.
Published: (2006-03-01) -
Evaluation of a kDNA-Based qPCR Assay for the Detection and Quantification of Old World <i>Leishmania</i> Species
by: Marcello Ceccarelli, et al.
Published: (2020-12-01)