Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts.

BACKGROUND:The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain seg...

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Bibliographic Details
Main Authors: Renata de Cássia-Pires, Myllena de Fátima Alheiros Dias de Melo, Raquel da Hora Barbosa, André Luiz Rodrigues Roque
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5354409?pdf=render

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